Balfagón G, Galván R, Marco E J
J Pharm Pharmacol. 1984 Apr;36(4):248-52. doi: 10.1111/j.2042-7158.1984.tb04359.x.
KCl (50 mM), tyramine (10(-7) M), and histamine (10(-4) M) induced an increase in tritium release from cat cerebral arteries preincubated with [3H]noradrenaline, this increase being due in part to noradrenaline. When calcium was absent from the superfusion medium, only tyramine (10(-7) M) enhanced the tritium outflow. Colchicine (10(-3) M) partially inhibited the increase in radioactivity brought about by 10(-4) M histamine. KCl (50 mM) also evoked release of radioactivity from cerebral arteries preloaded with [3H]histamine; this release was unaffected by reserpine pretreatment or removal of both superior cervical sympathetic ganglia. Neither tyramine (10(-7) M) nor compound 48/80 (300 micrograms ml-1) altered the spontaneous tritium outflow from cerebral blood vessels preincubated with [3H]histamine. These results suggest that histamine is not accumulated by sympathetic nerve endings and elicits its noradrenaline-releasing effect by means of an exocytotic process.
氯化钾（50毫摩尔）、酪胺（10⁻⁷摩尔）和组胺（10⁻⁴摩尔）可使预先用[³H]去甲肾上腺素孵育的猫脑动脉中氚释放增加，这种增加部分归因于去甲肾上腺素。当灌流介质中无钙时，只有酪胺（10⁻⁷摩尔）能增强氚流出。秋水仙碱（10⁻³摩尔）部分抑制了10⁻⁴摩尔组胺引起的放射性增加。氯化钾（50毫摩尔）也能促使预先加载[³H]组胺的脑动脉释放放射性；这种释放不受利血平预处理或双侧颈上交感神经节切除的影响。酪胺（10⁻⁷摩尔）和48/80化合物（300微克/毫升）均未改变预先用[³H]组胺孵育的脑血管的自发氚流出。这些结果表明，组胺不被交感神经末梢摄取，而是通过胞吐过程发挥其去甲肾上腺素释放作用。
