Dennis R
J Pharm Pharmacol. 1984 May;36(5):332-3. doi: 10.1111/j.2042-7158.1984.tb04386.x.
Strychnine is separated from other alkaloids in nux vomica tincture in less than 6 min on a 12.5 cm Hypersil column using a mobile phase of methanol, 2 M ammonium hydroxide, M ammonium nitrate (27:2:1), uv detection at 254 nm. Quantitative estimation may be obtained by comparing peak area or height against an external standard (0.150% w/v strychnine base in 45% ethanol). The results obtained were comparable to those obtained by the BP method which takes 5 h.
在一根12.5厘米的Hypersil柱上，使用甲醇、2M氢氧化铵、M硝酸铵（27:2:1）的流动相，在254纳米处进行紫外检测，士的宁在不到6分钟内即可从马钱子酊剂中的其他生物碱中分离出来。通过将峰面积或峰高与外标（45%乙醇中0.150%w/v士的宁碱）进行比较，可获得定量估计值。所得结果与采用BP方法（耗时5小时）获得的结果相当。
