Agosin M, Cherry A, Pedemonte J, White R
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(1):127-32. doi: 10.1016/0742-8413(84)90058-6.
Trypanosoma cruzi epimastigote and trypomastigote forms contain microsomal peptides in the 40-60,000 mol. wt region, some of which are heme-staining-positive and are induced by phenobarbital, as indicated by SDS-gel electrophoresis and by double-labeling experiments. Epimastigotes show induced peptides of mol. wt 56,000, 52,000, 49,000, 44,000, 42,000 and 40,500 whereas only one peptide (52,500 mol. wt) is increased in trypomastigotes. Fractionation of microsomes derived from epimastigotes by octylamine Sepharose-4B column chromatography reveals the presence of two heme peptides with mol. wt of 55,800 and 56,600. The pooled fraction has a typical cytochrome P-450 CO-difference spectrum and appears to correspond to a high spin form. The demonstration of the existence of this family of hemoproteins in T. cruzi further supports the idea that resistance to chemotherapeutic agents is due to active metabolism. The active metabolism, however, may not be similar in the various developmental forms of this organism since differences exist in the patterns of induction of heme-positive microsomal peptides.
克氏锥虫的上鞭毛体和锥鞭毛体形式在分子量40,000 - 60,000区域含有微粒体肽，其中一些经十二烷基硫酸钠凝胶电泳和双标记实验表明，是由苯巴比妥诱导产生的、呈血红素染色阳性的肽。上鞭毛体显示出分子量为56,000、52,000、49,000、44,000、42,000和40,500的诱导肽，而锥鞭毛体中仅一种肽（分子量52,500）有所增加。通过辛胺琼脂糖-4B柱色谱法对源自上鞭毛体的微粒体进行分级分离，发现存在两种分子量分别为55,800和56,600的血红素肽。合并后的级分具有典型的细胞色素P - 450一氧化碳差光谱，似乎对应于一种高自旋形式。在克氏锥虫中证明这类血红蛋白的存在，进一步支持了对化疗药物产生抗性是由于活跃代谢这一观点。然而，由于血红素阳性微粒体肽的诱导模式存在差异，这种生物体的不同发育形式中的活跃代谢可能并不相同。
