Sheumack D D, Baldo B A, Carroll P R, Hampson F, Howden M E, Skorulis A
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(1):55-68. doi: 10.1016/0742-8413(84)90048-3.
The crude venoms of male and female Sydney funnel web spiders, Atrax robustus, were compared by cation-exchange and high-performance liquid chromatography, lethality to new-born mice, polyacrylamide gel isoelectric focusing, immunoelectrophoresis, phospholipase A analysis, effects on the mouse phrenic nerve hemidiaphragm and passive paw oedema in the rat and, except in the case of rat paw oedema, were found to exhibit quite different properties. Polyacrylamide gel isoelectric focusing and high-performance liquid chromatography proved to be suitable for gender and species determination when applied to the venoms of A. formidabilis, A. infensus, A. robustus and A. versutus. These venoms were also compared by lethality, promotion of muscle fasciculation and phospholipase A activity.
通过阳离子交换和高效液相色谱法、对新生小鼠的致死性、聚丙烯酰胺凝胶等电聚焦法、免疫电泳法、磷脂酶A分析、对小鼠膈神经半膈肌的影响以及对大鼠被动爪肿胀的影响，对雄性和雌性悉尼漏斗网蜘蛛（Atrax robustus）的粗毒液进行了比较，结果发现，除了对大鼠爪肿胀的影响外，它们表现出截然不同的特性。聚丙烯酰胺凝胶等电聚焦法和高效液相色谱法在应用于A. formidabilis、A. infensus、A. robustus和A. versutus的毒液时，被证明适用于性别和物种的鉴定。还通过致死性、促进肌肉束颤和磷脂酶A活性对这些毒液进行了比较。
