漏斗网蜘蛛Atrax versutus毒液中的致命神经毒素——强效毒素的氨基酸序列。
Amino acid sequence of versutoxin, a lethal neurotoxin from the venom of the funnel-web spider Atrax versutus.
作者信息
Brown M R, Sheumack D D, Tyler M I, Howden M E
机构信息
School of Chemistry, Macquarie University, Sydney, N.S.W., Australia.
出版信息
Biochem J. 1988 Mar 1;250(2):401-5. doi: 10.1042/bj2500401.
Abstract
The complete amino acid sequence of versutoxin, a lethal neurotoxic polypeptide isolated from the venom of male and female funnel-web spiders of the species Atrax versutus, was determined. Sequencing was performed in a gas-phase protein sequencer by automated Edman degradation of the S-carboxymethylated toxin and fragments of it produced by reaction with CNBr. Versutoxin consisted of a single chain of 42 amino acid residues. It was found to have a high proportion of basic residues and of cystine. The primary structure showed marked homology with that of robustoxin, a novel neurotoxin recently isolated from the venom of another funnel-web-spider species, Atrax robustus.
摘要
从雄性和雌性剧毒漏斗网蜘蛛（Atrax versutus）毒液中分离出的一种致命神经毒性多肽——威猛毒素（versutoxin）的完整氨基酸序列已被确定。通过在气相蛋白质测序仪中对S-羧甲基化毒素及其与溴化氰反应产生的片段进行自动埃德曼降解来进行测序。威猛毒素由一条含42个氨基酸残基的单链组成。发现它含有高比例的碱性残基和胱氨酸。其一级结构与最近从另一种漏斗网蜘蛛物种——粗壮漏斗网蜘蛛（Atrax robustus）毒液中分离出的新型神经毒素——强壮毒素（robustoxin）的一级结构有显著同源性。
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本文引用的文献
1
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2
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3
Human liver alcohol dehydrogenase. 1. The primary structure of the beta 1 beta 1 isoenzyme.人肝脏乙醇脱氢酶。1. β1β1同工酶的一级结构。
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4
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5
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6
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