Sutherland S K
Med J Aust. 1980 Oct 18;2(8):437-41. doi: 10.5694/j.1326-5377.1980.tb131913.x.
A purified rabbit IgG (antivenom) has been isolated from rabbits immunized with male Atrax robustus venom. This preparation has been shown to neutralize in vitro, male venom in a ratio of 97 microgram of IgG to 1 microgram of male venom. The venoms of a number of other members of the Atrax genus are also neutralized in vitro by this antivenom. Of particular importance is the fact that the venom of the extremely dangerous female A. formidabilis is neutralized. A serum harvest of 55 mL from an immunized rabbit yielded a total of 1.1869 g of immune IgG using the Protein A-Sepharose procedure. This quantity is sufficient antivenom to neutralize in vitro the average yield of 67 spiders. These findings suggest the preparation of an antivenom for human use is now feasible. The antivenom was shown to effectively neutralize venom in monkeys either when it was premixed with the venom before injection or when it was injected separately 10 minutes after injection of venom. This is the first time in-vitro and in-vivo neutralization of this venom has been demonstrated in the monkey. In other studies, a range of non-immunized animal sera was shown to have no inherent ability to neutralize male A. robustus venom in vitro.
一种纯化的兔免疫球蛋白（抗蛇毒血清）已从用雄性粗壮漏斗蛛毒液免疫的兔子中分离出来。该制剂已被证明在体外能以97微克免疫球蛋白中和1微克雄性毒液的比例中和雄性毒液。这种抗蛇毒血清在体外也能中和许多其他漏斗蛛属成员的毒液。特别重要的是，极其危险的雌性可怕漏斗蛛的毒液也能被中和。使用蛋白A-琼脂糖凝胶法，从一只免疫兔子身上采集55毫升血清，共获得1.
