Takasaki Y, Robinson W A, Tan E M
J Natl Cancer Inst. 1984 Sep;73(3):655-61.
A nuclear antigen associated with cell proliferation [proliferating cell nuclear antigen (PCNA)] and blast transformation is recognized by autoantibodies in the sera of some patients with systemic lupus erythematosus; these autoantibodies are precipitating antibodies and also react in immunofluorescence, a technique that was used to determine if PCNA might be expressed in leukocytes of patients with chronic myeloid leukemia (CML) during certain phases of their disease. At all times, a strong relationship was seen between the percentage of cells stained by anti-PCNA antibody and the percentage of blast cells in peripheral blood leukocytes (r) = 0.865, P less than .001. However, during blast crisis, certain cells that morphologically looked like myelocytes, metamyelocytes, and some cells with segmented nuclei stained with anti-PCNA serum. This staining, which remained nuclear in location, was less intense than in blast cells, suggesting low density of the antigen in these nonblast cells. This phenomenon was not observed in myelocytes or metamyelocytes obtained from patients in remission. These initial studies demonstrated that anti-PCNA can be used as a reagent to detect blast cells in CML crisis and also has the capability to detect PCNA in other cells associated with blast crisis.
一种与细胞增殖相关的核抗原[增殖细胞核抗原（PCNA）]和原始细胞转化可被一些系统性红斑狼疮患者血清中的自身抗体识别；这些自身抗体是沉淀抗体，也可在免疫荧光中发生反应，免疫荧光技术曾被用于确定PCNA是否可能在慢性粒细胞白血病（CML）患者疾病的某些阶段在其白细胞中表达。在所有时间点，抗PCNA抗体染色的细胞百分比与外周血白细胞中的原始细胞百分比之间均呈现出很强的相关性（r = 0.865，P < 0.001）。然而，在原始细胞危象期间，某些形态上看起来像髓细胞、晚幼粒细胞的细胞以及一些具有分叶核的细胞被抗PCNA血清染色。这种染色在位置上仍位于细胞核，但其强度低于原始细胞，表明这些非原始细胞中抗原密度较低。在缓解期患者的髓细胞或晚幼粒细胞中未观察到这种现象。这些初步研究表明，抗PCNA可作为一种试剂用于检测CML危象中的原始细胞，并且还具有检测与原始细胞危象相关的其他细胞中PCNA的能力。
