Bujia J, Sudhoff H, Holly A, Hildmann H, Kastenbauer E
Department of Otorhinolaryngology, Ludwid-Maximilians-University, Klinikum Grobhadern, Munich, Germany.
Eur Arch Otorhinolaryngol. 1996;253(1-2):21-4. doi: 10.1007/BF00176697.
Cholesteatoma epithelium is characterized by a keratinocyte dysregulation accompanied by destruction of the ossicles and temporal bone. Immunohistochemical methods using antibodies to cell-cycle-related antigens can be used as a means for assessing various aspects of proliferation in cholesteatoma tissue. They also have the important advantage of preserving the spatial orientation of proliferating cells in histological sections. Proliferating cell nuclear antigen (PCNA) is a 36 kDa DNA-delta-polymerase-associated protein that is directly involved in the mechanisms of DNA synthesis. In the present study the expression of PCNA was investigated in formalin-fixed, paraffin-embedded biopsy specimens of cholesteatomas and normal skin. Normal skin revealed nuclear staining in a small number of keratinocytes (PCNA grade, 1.5) located in the basal cell layer. In contrast, an increased number of PCNA-labeled basal and suprabasal epidermal cells (PCNA grade, 9.3) were found in cholesteatoma samples. Our findings indicate that PCNA represents a reliable marker for epithelial proliferation, showing that cholesteatoma epithelium proliferates at a higher rate than normal epidermis. These findings also support the concept of keratinocyte dysregulation in middle ear cholesteatoma.
胆脂瘤上皮的特征是角质形成细胞失调，并伴有听小骨和颞骨的破坏。使用针对细胞周期相关抗原的抗体的免疫组织化学方法可作为评估胆脂瘤组织增殖各个方面的手段。它们还具有在组织学切片中保留增殖细胞空间取向的重要优势。增殖细胞核抗原（PCNA）是一种36 kDa的与DNAδ聚合酶相关的蛋白质，直接参与DNA合成机制。在本研究中，对胆脂瘤和正常皮肤的福尔马林固定、石蜡包埋活检标本中PCNA的表达进行了研究。正常皮肤在位于基底细胞层的少数角质形成细胞中显示核染色（PCNA分级，1.5）。相比之下，在胆脂瘤样本中发现PCNA标记的基底和基底上层表皮细胞数量增加（PCNA分级，9.3）。我们的研究结果表明，PCNA是上皮增殖的可靠标志物，表明胆脂瘤上皮的增殖速率高于正常表皮。这些发现也支持中耳胆脂瘤中角质形成细胞失调的概念。
