Lafranconi W M, Ferlan I, Russell F E, Huxtable R J
Toxicon. 1984;22(3):347-52. doi: 10.1016/0041-0101(84)90078-3.
On perfusion through isolated lungs from male Sprague-Dawley rats, equinatoxin caused a dose-dependent increase in the wet to dry weight ratio. Ratios were significantly elevated above control values at equinatoxin concentrations of 80-200 ng/ml. The increased ratios were accompanied by an increase in the permeability of the lung vasculature. When equinatoxin was perfused through isolated lungs at concentrations of 100 ng/ml or greater, significantly more [3H]polyethylene glycol (PEG; approximately 900 mol. wt) was retained in the extravascular space as compared to controls. Perfusion pressures of the lung were significantly elevated above controls at equinatoxin concentrations greater than 100 ng/ml. These effects of equinatoxin were not mediated by degranulation of mast cells, as preperfusion of the lung with 100 or 200 microM Na cromolyn or 1 microM lanthanum chloride did not modify the pulmonary response to equinatoxin. At concentrations of equinatoxin below 150 ng/ml the fluid movement appears to be restricted primarily to intracellular, or possibly interstitial, spaces, as no significant amounts of [3H]polyethylene glycol were recovered by tracheal lavage. At concentrations of equinatoxin equal to or greater than 150 ng/ml, significant amounts of PEG were washed from the trachea. As it is a potent inducer of pulmonary edema, equinatoxin may become an important probe to study fluid regulation in the lung.
在对雄性斯普拉格-道利大鼠的离体肺进行灌注时，海葵毒素导致湿重与干重之比呈剂量依赖性增加。在海葵毒素浓度为80 - 200 ng/ml时，该比值显著高于对照值。比值升高伴随着肺血管通透性增加。当以100 ng/ml或更高浓度的海葵毒素灌注离体肺时，与对照组相比，血管外间隙中保留的[3H]聚乙二醇（PEG；分子量约900）显著更多。在海葵毒素浓度大于100 ng/ml时，肺的灌注压力显著高于对照组。海葵毒素的这些作用不是由肥大细胞脱颗粒介导的，因为用100或200 μM色甘酸钠或1 μM氯化镧预先灌注肺并没有改变肺对海葵毒素的反应。在海葵毒素浓度低于150 ng/ml时，液体移动似乎主要局限于细胞内或可能的间质空间，因为气管灌洗未回收大量的[3H]聚乙二醇。在海葵毒素浓度等于或大于150 ng/ml时，有大量PEG从气管中被冲洗出来。由于海葵毒素是肺水肿的强效诱导剂，它可能成为研究肺内液体调节的重要探针。
