用于枯草芽孢杆菌的单一化学限定芽孢形成培养基：生长、芽孢形成及细胞外蛋白酶产生
Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production.
作者信息
Hageman J H, Shankweiler G W, Wall P R, Franich K, McCowan G W, Cauble S M, Grajeda J, Quinones C
出版信息
J Bacteriol. 1984 Oct;160(1):438-41. doi: 10.1128/jb.160.1.438-441.1984.
Abstract
The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid production was completed between 7 and 11 h. Cells grown in the single, chemically defined medium excreted levels of serine and neutral proteases comparable to those excreted in nutrient broth medium.
摘要
描述了一种单一的、化学成分明确的培养基的组成及其在枯草芽孢杆菌生长和孢子形成中的应用。在37℃下，细胞以约40分钟的倍增时间生长；培养物在指数生长结束后12小时达到接近最大的孢子形成（70%至80%），并在24小时产生1×10⁹至2×10⁹个耐热游离孢子。吡啶二羧酸的产生在7至11小时之间完成。在单一的化学成分明确的培养基中生长的细胞分泌的丝氨酸和中性蛋白酶水平与在营养肉汤培养基中分泌的相当。
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