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J Bacteriol. 1984 Oct;160(1):438-41. doi: 10.1128/jb.160.1.438-441.1984.
2
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Sporulation in Bacillus subtilis. Biochemical changes.枯草芽孢杆菌中的孢子形成。生化变化。
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Bacitracin and protease production in relation to sporulation during exponential growth of Bacillus licheniformis on poorly utilized carbon and nitrogen sources.地衣芽孢杆菌在利用不良的碳源和氮源上指数生长期间，杆菌肽和蛋白酶产生与芽孢形成的关系。
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本文引用的文献

1
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.脱氧核糖核酸对枯草芽孢杆菌生化缺陷菌株的转化
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8. doi: 10.1073/pnas.44.10.1072.
2
CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS.用于枯草芽孢杆菌芽孢形成、萌发及生长的化学成分确定的合成培养基。
J Bacteriol. 1964 Feb;87(2):332-6. doi: 10.1128/jb.87.2.332-336.1964.
3
Colorimetric assay for dipicolinic acid in bacterial spores.细菌芽孢中吡啶二羧酸的比色测定法。
Science. 1958 Jan 3;127(3288):26-7. doi: 10.1126/science.127.3288.26.
4
Enzyme changes during Bacillus subtilis sporulation caused by deprivation of guanine nucleotides.鸟嘌呤核苷酸缺乏导致枯草芽孢杆菌孢子形成过程中的酶变化。
J Bacteriol. 1980 Dec;144(3):1119-25. doi: 10.1128/jb.144.3.1119-1125.1980.
5
Involvement of the stringent response in degradation of glutamine phosphoribosylpyrophosphate amidotransferase in Bacillus subtilis.枯草芽孢杆菌中严格反应参与谷氨酰胺磷酸核糖焦磷酸酰胺转移酶的降解
J Bacteriol. 1983 Jul;155(1):56-63. doi: 10.1128/jb.155.1.56-63.1983.
6
Regulation of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase inactivation in vivo.体内枯草芽孢杆菌谷氨酰胺磷酸核糖焦磷酸酰胺转移酶失活的调控
J Bacteriol. 1983 Feb;153(2):937-49. doi: 10.1128/jb.153.2.937-949.1983.
7
Nutritional regulation of degradation of aspartate transcarbamylase and of bulk protein in exponentially growing Bacillus subtilis cells.指数生长的枯草芽孢杆菌细胞中天冬氨酸转氨甲酰酶及大量蛋白质降解的营养调控
J Bacteriol. 1983 Jan;153(1):253-8. doi: 10.1128/jb.153.1.253-258.1983.
8
Replacement sporulation of Bacillus subtilis 168 in a chemically defined medium.枯草芽孢杆菌168在化学限定培养基中的替代孢子形成
J Bacteriol. 1970 Jan;101(1):1-8. doi: 10.1128/jb.101.1.1-8.1970.
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Sporulation in Bacillus subtilis. The role of exoprotease.枯草芽孢杆菌中的孢子形成。胞外蛋白酶的作用。
Biochem J. 1968 Oct;109(5):793-801. doi: 10.1042/bj1090793.
10
Biochemical studies of bacterial sporulation and germaination. VII. Protein turnover during sporulation of Bacillus subtilis.细菌孢子形成与萌发的生化研究。VII. 枯草芽孢杆菌孢子形成过程中的蛋白质周转
J Biol Chem. 1968 Sep 10;243(17):4600-5.

用于枯草芽孢杆菌的单一化学限定芽孢形成培养基：生长、芽孢形成及细胞外蛋白酶产生

Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production.

作者信息

Hageman J H, Shankweiler G W, Wall P R, Franich K, McCowan G W, Cauble S M, Grajeda J, Quinones C

出版信息

J Bacteriol. 1984 Oct;160(1):438-41. doi: 10.1128/jb.160.1.438-441.1984.

DOI:10.1128/jb.160.1.438-441.1984
PMID:6148336
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214739/
Abstract

The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid production was completed between 7 and 11 h. Cells grown in the single, chemically defined medium excreted levels of serine and neutral proteases comparable to those excreted in nutrient broth medium.

摘要

描述了一种单一的、化学成分明确的培养基的组成及其在枯草芽孢杆菌生长和孢子形成中的应用。在37℃下，细胞以约40分钟的倍增时间生长；培养物在指数生长结束后12小时达到接近最大的孢子形成（70%至80%），并在24小时产生1×10⁹至2×10⁹个耐热游离孢子。吡啶二羧酸的产生在7至11小时之间完成。在单一的化学成分明确的培养基中生长的细胞分泌的丝氨酸和中性蛋白酶水平与在营养肉汤培养基中分泌的相当。