枯草芽孢杆菌中的孢子形成。生化变化。

Sporulation in Bacillus subtilis. Biochemical changes.

作者信息

Warren S C

出版信息

Biochem J. 1968 Oct;109(5):811-8. doi: 10.1042/bj1090811.

DOI:10.1042/bj1090811

PMID:4972255

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1187032/

Abstract

During the course of growth and sporulation of Bacillus subtilis in chemically defined media, measurements were made of 16 different parameters, including the specific activities of nine intracellular enzymes. 2. Towards the end of exponential growth, proteolytic activity increased and reached a maximum soon after growth ceased. 3. In the presence of an excess of phosphate the specific activity of alkaline phosphatase increased fivefold at the end of exponential growth. 4. The specific activity of malate dehydrogenase remained at a high constant level throughout sporulation, but the specific activity of fumarase showed a two- to three-fold increase 5-9hr. after the end of exponential growth. 5. Aconitase activity was barely detectable during exponential growth in a glucose-glutamate medium, but increased rapidly when glutamate was replaced by citrate or when the glucose in the medium was exhausted. 6. The specific activity of alanine dehydrogenase increased threefold 1-5hr. after the end of exponential growth. 7. The specific activity of soluble NADH oxidase doubled 4-6hr. after the end of exponential growth. 8. Glucose dehydrogenase was undetectable until 4hr. after the end of exponential growth, but its specific activity increased 20-fold over the next 3-4hr. 9. The onset of refractility, the synthesis of 2,6-dipicolinic acid and the appearance of heat-resistance occurred in this order some 6-12hr. after the end of exponential growth. 10. The significance of these changes is discussed in relation to the morphological development of the spore.

摘要

在枯草芽孢杆菌于化学成分确定的培养基中生长和形成芽孢的过程中，对16个不同参数进行了测量，包括9种细胞内酶的比活性。2. 在指数生长接近尾声时，蛋白水解活性增加，并在生长停止后不久达到最大值。3. 在磷酸盐过量的情况下，碱性磷酸酶的比活性在指数生长结束时增加了五倍。4. 苹果酸脱氢酶的比活性在整个芽孢形成过程中保持在较高的恒定水平，但延胡索酸酶的比活性在指数生长结束后5 - 9小时增加了两到三倍。5. 在葡萄糖 - 谷氨酸培养基的指数生长期间，乌头酸酶活性几乎检测不到，但当谷氨酸被柠檬酸盐取代或培养基中的葡萄糖耗尽时，其活性迅速增加。6. 丙氨酸脱氢酶的比活性在指数生长结束后1 - 5小时增加了三倍。7. 可溶性NADH氧化酶的比活性在指数生长结束后4 - 6小时增加了一倍。8. 葡萄糖脱氢酶直到指数生长结束后4小时才被检测到，但其比活性在接下来的3 - 4小时内增加了20倍。9. 折光性的开始、2,6 - 二吡啶甲酸的合成以及耐热性的出现大约在指数生长结束后6 - 12小时按此顺序发生。10. 结合芽孢的形态发育对这些变化的意义进行了讨论。