枯草芽孢杆菌孢子形成过程中丝氨酸蛋白酶的产生及可能的功能
Production and possible function of serine protease during sporulation of Bacillus subtilis.
作者信息
Dancer B N, Mandelstam J
出版信息
J Bacteriol. 1975 Feb;121(2):406-10. doi: 10.1128/jb.121.2.406-410.1975.
Abstract
The production of extracellular protease during sporulation in Bacillus subtilis 168 was investigated. Two proteases are produced, an alkaline serine protease and a neutral metalloprotease. In vivo inhibition of the serine protease with phenylmethylsulfonylfluoride indicated that the metalloprotease was degraded by the serine protease during sporulation. The experiments with phenylmethylsulfonylfluoride also show that the serine protease is necessary for the sequential process of sporulation and that it is required continuously for the first 2 to 3 h of the 8-h process.
摘要
对枯草芽孢杆菌168在芽孢形成过程中细胞外蛋白酶的产生进行了研究。产生了两种蛋白酶，一种碱性丝氨酸蛋白酶和一种中性金属蛋白酶。用苯甲基磺酰氟在体内抑制丝氨酸蛋白酶表明，在芽孢形成过程中金属蛋白酶被丝氨酸蛋白酶降解。用苯甲基磺酰氟进行的实验还表明，丝氨酸蛋白酶对于芽孢形成的连续过程是必需的，并且在8小时过程的前2至3小时内持续需要它。
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