Kristiansen B E, Sørensen B, Simonsen T, Spanne O, Lund V, Bjorvatn B
J Infect Dis. 1984 Sep;150(3):389-96. doi: 10.1093/infdis/150.3.389.
Neisseria meningitidis was isolated from the throat, blood, and cerebrospinal fluid of six patients and from the throat and blood of two patients. All 22 isolates were of serogroup B, serotype 15, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis type IV. Isolates from a single patient always possessed identical DNA restriction endonuclease patterns; this observation strongly suggested genomic identity. In spite of this apparent genomic identity, however, significant differences in piliation and adherence were observed among isolates from different sites in the same patient. Isolates from the throat were significantly more piliated (P less than .001) and adhered to human buccal epithelial cells in vitro in significantly higher numbers (P less than .001) than did isolates from the blood and the cerebrospinal fluid of the same patient. These results indicate that phase shift occurs in N. meningitidis in vivo.
从6名患者的咽喉、血液和脑脊液以及2名患者的咽喉和血液中分离出脑膜炎奈瑟菌。所有22株分离株均为B血清群、15血清型，且十二烷基硫酸钠-聚丙烯酰胺凝胶电泳为IV型。来自同一患者的分离株总是具有相同的DNA限制性内切酶图谱；这一观察结果强烈表明基因组相同。然而，尽管有这种明显的基因组相同性，但在同一患者不同部位的分离株之间观察到菌毛形成和黏附存在显著差异。与同一患者血液和脑脊液中的分离株相比，咽喉部位的分离株菌毛显著更多（P<0.001），并且在体外黏附于人颊黏膜上皮细胞的数量显著更高（P<0.001）。这些结果表明脑膜炎奈瑟菌在体内会发生相转变。
