Yakubu D E, Abadi F J, Pennington T H
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill.
Epidemiol Infect. 1994 Aug;113(1):53-65. doi: 10.1017/s0950268800051463.
The genomes of 34 recent United Kingdom isolates of Neisseria meningitidis serogroup C were examined by restriction enzyme digestion and by conventional and pulsed-field gel electrophoresis (PFGE). Strains were assigned to groups on the basis of the Dice similarity coefficient; strains with values of > 92% were considered to be clonally related. Twelve clones were identified by PFGE. Strains of two clonal groups predominated. Restriction endonuclease analyses using the 'high frequency cleavage' endonuclease Stu I and conventional electrophoresis gave 11 groups; in general it had lower resolving power than PFGE.
通过限制性酶切消化以及常规和脉冲场凝胶电泳（PFGE）对34株近期分离自英国的C群脑膜炎奈瑟菌的基因组进行了检测。根据Dice相似系数将菌株分组；相似系数值>92%的菌株被认为具有克隆相关性。通过PFGE鉴定出12个克隆。两个克隆群的菌株占主导地位。使用“高频切割”内切酶Stu I和常规电泳进行的限制性内切酶分析得出11个组；总体而言，其分辨能力低于PFGE。
