Kristiansen B E, Sørensen B, Bjorvatn B, Falk E S, Fosse E, Bryn K, Frøholm L O, Gaustad P, Bøvre K
J Clin Microbiol. 1986 Apr;23(4):764-7. doi: 10.1128/jcm.23.4.764-767.1986.
Following an outbreak of meningococcal disease in three schoolchildren in a small community in northern Norway, DNA fingerprinting, serotyping with monoclonal antibodies, serogrouping, and sulfonamide sensitivity testing were applied for characterization and tracing of the causative agent. The three case isolates were genomically indistinguishable, sulfonamide-resistant, serogroup B, serotype 15 meningococci. Throat specimens were collected from 552 healthy contacts, including all children below age 17 and their parents. Among the 36 carrier isolates (carrier rate, 6.5%) 13 showed DNA fingerprints identical, or almost identical, to the index pattern. All of these 13 isolates were sulfonamide resistant, 12 were of serotype 15, and 8 were of polysaccharide serogroup B (5 were nongroupable). These closely related isolates were almost exclusively recovered from schoolchildren of 2 of 15 small villages, one of which included the homes of two of the patients. The remaining 23 carrier isolates were nonresistant, non-type 15 meningococci of widely differing DNA restriction patterns. Our results confirm that DNA fingerprinting has potential as an efficient tool in practical meningococcal epidemiology.
在挪威北部一个小社区的三名学童中爆发脑膜炎球菌病后，采用了DNA指纹图谱分析、单克隆抗体血清分型、血清群鉴定和磺胺类药物敏感性试验来鉴定和追踪病原体。三例病例分离株在基因组上无法区分，对磺胺类药物耐药，属于B血清群、15型脑膜炎球菌。从552名健康接触者中采集了咽喉样本，包括所有17岁以下儿童及其父母。在36例带菌者分离株中（带菌率为6.5%），有13株的DNA指纹图谱与索引图谱相同或几乎相同。这13株分离株均对磺胺类药物耐药，12株为15型，8株为多糖B血清群（5株无法分型）。这些密切相关的分离株几乎全部从15个小村庄中的2个村庄的学童中分离得到，其中一个村庄包括两名患者的家。其余23例带菌者分离株对磺胺类药物不耐药，是DNA限制性图谱差异很大的非15型脑膜炎球菌。我们的结果证实，DNA指纹图谱分析在实际的脑膜炎球菌流行病学中具有作为有效工具的潜力。
