Hösli L, Hösli E, Schneider U, Wiget W
Neurosci Lett. 1984 Aug 10;48(3):287-91. doi: 10.1016/0304-3940(84)90052-1.
By means of intracellular microelectrodes we have studied the action of histamine H1- and H2-agonists and -antagonists on the membrane potential of astrocytes in cultured rat brainstem and spinal cord. Histamine at high concentrations (10(-4) and 10(-5) M) mainly depolarized the glial membrane, whereas at low concentration (10(-6) M) it usually caused hyperpolarizations. The histamine-induced depolarizations were reversibly blocked by the H1-antagonist pyrilamine, whereas the H2-antagonist cimetidine antagonized the hyperpolarizations. The H1-agonist thiazolethylamine mainly produced depolarizations while impromidine, a H2-agonist, predominantly caused hyperpolarizations. Our findings, together with autoradiographic binding studies, provide strong evidence for the existence of histamine H1- and H2-receptors on astrocytes.
我们通过细胞内微电极研究了组胺H1和H2激动剂及拮抗剂对培养的大鼠脑干和脊髓星形胶质细胞膜电位的作用。高浓度（10⁻⁴和10⁻⁵ M）的组胺主要使胶质膜去极化，而低浓度（10⁻⁶ M）时通常引起超极化。组胺诱导的去极化可被H1拮抗剂吡苄明可逆性阻断，而H2拮抗剂西咪替丁则拮抗超极化。H1激动剂噻唑乙胺主要产生去极化，而H2激动剂英普咪定则主要引起超极化。我们的研究结果与放射自显影结合研究一起，为星形胶质细胞上存在组胺H1和H2受体提供了有力证据。
