Donaldson D J, Mahan J T
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(2):267-70. doi: 10.1016/0742-8413(84)90081-1.
Following removal of a skin patch from each hind limb of a series of adult newts, the limbs were explanted into small dishes of Holtfreter solution containing various combinations of test drugs. Later, the amount of wound epithelium that formed on each limb was determined using a planimeter on wound tracings obtained with the aid of a drawing tube-equipped microscope. All three (-)-catecholamines tested inhibited migration with the following order of effectiveness: isoproterenol greater than epinephrine greater than norepinephrine. The effect was stereospecific with (-)-isoproterenol clearly more effective than the (+)-isomer. Propranolol, a beta antagonist, blocked the effect of (-)-isoproterenol while the alpha antagonist, phentolamine, was less effective. One hour in (-)-isoproterenol inhibited migration to the same degree as continuous exposure for the 6 hr most experiments were allowed to run. Taken together, the data suggest that catecholamines exert their effect on epidermal cell migration via beta 2 adrenergic receptors on the cell surface, a binding site which implicates cAMP in the chain of events.
在从一系列成年蝾螈的每个后肢移除皮肤贴片后，将这些肢体移植到含有各种测试药物组合的小培养皿中的霍尔特弗雷特溶液中。之后，使用配备绘图管的显微镜获得伤口描图，再用求积仪测定每个肢体上形成的伤口上皮的量。所测试的所有三种（-）-儿茶酚胺均抑制迁移，其效力顺序如下：异丙肾上腺素大于肾上腺素大于去甲肾上腺素。该效应具有立体特异性，（-）-异丙肾上腺素明显比（+）-异构体更有效。β拮抗剂普萘洛尔可阻断（-）-异丙肾上腺素的作用，而α拮抗剂酚妥拉明的效果较差。在（-）-异丙肾上腺素中处理一小时与大多数实验允许进行的6小时连续暴露抑制迁移的程度相同。综合来看，数据表明儿茶酚胺通过细胞表面的β2肾上腺素能受体对表皮细胞迁移发挥作用，该结合位点表明环磷酸腺苷参与了这一系列事件。
