大鼠脑活体的同核¹H双共振差谱学
Homonuclear 1H double-resonance difference spectroscopy of the rat brain in vivo.
作者信息
Rothman D L, Behar K L, Hetherington H P, Shulman R G
出版信息
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6330-4. doi: 10.1073/pnas.81.20.6330.
Abstract
We have used 1H homonuclear double-resonance difference spectroscopy at 360.13 MHz to resolve specific metabolite resonances in the brains of intact rats. Metabolite resonances resolved include previously obscured proton resonances of alanine, gamma-aminobutyric acid (GABA), glutamate, and taurine. The gamma-aminobutyric acid alpha- and gamma-CH2 proton resonances were observed in the living rat in the difference spectrum obtained upon irradiation of the beta-CH2 proton resonance at 1.91 ppm. A 3-fold increase in the intensity of the alpha- and gamma-CH2 resonances of gamma-aminobutyric acid was observed 30 min after death. The alpha-CH and gamma-CH2 resonances of glutamate were also resolved in vivo by selective irradiation of the beta-CH2 protons to which they are spin-coupled. In addition, this technique was used to observe the beta-CH3 protons of lactate through the intact scalp of a rat. Large lipid signals arising from scalp tissue were eliminated in the difference spectrum, revealing the lactate beta-CH3 resonance.
摘要
我们使用了360.13兆赫兹的1H同核双共振差谱技术来解析完整大鼠大脑中特定代谢物的共振信号。解析出的代谢物共振信号包括先前被掩盖的丙氨酸、γ-氨基丁酸（GABA）、谷氨酸和牛磺酸的质子共振信号。在1.91 ppm处照射β-CH2质子共振信号后获得的差谱中，在活体大鼠中观察到了γ-氨基丁酸的α-和γ-CH2质子共振信号。死亡30分钟后，观察到γ-氨基丁酸的α-和γ-CH2共振信号强度增加了3倍。通过选择性照射与谷氨酸的α-CH和γ-CH2共振信号自旋耦合的β-CH2质子，也在体内解析出了谷氨酸的α-CH和γ-CH2共振信号。此外，该技术还用于通过大鼠完整的头皮观察乳酸的β-CH3质子。头皮组织产生的大量脂质信号在差谱中被消除，从而揭示了乳酸的β-CH3共振信号。
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