体内脑缺氧的高分辨率1H核磁共振研究。
High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo.
作者信息
Behar K L, den Hollander J A, Stromski M E, Ogino T, Shulman R G, Petroff O A, Prichard J W
出版信息
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4945-8. doi: 10.1073/pnas.80.16.4945.
Abstract
1H NMR spectra at 360.13 MHz were obtained of the rat brain in vivo by using a surface coil placed over the skull. Resonances of numerous metabolites were identified by comparison with the 1H NMR spectra of excised rat brain tissue and acid extracts of the tissue. Changes in cerebral lactate levels resulting from the administration of gas mixtures low in oxygen were monitored in the in vivo brain spectra with a time resolution of 2.3 min. The electroencephalogram and electrocardiogram were recorded simultaneously during the NMR experiment. Reversibility of the hypoxic response was documented when, upon oxygen administration, cerebral lactate returned to its prehypoxic level. These experiments demonstrate the applicability of high-resolution 1H NMR to monitor pathophysiology of brain metabolism in real time.
摘要
通过将表面线圈置于大鼠颅骨上方，在体内获得了360.13 MHz的大鼠脑1H NMR谱。通过与切除的大鼠脑组织及其酸提取物的1H NMR谱进行比较，鉴定了多种代谢物的共振峰。在体内脑谱中监测了因给予低氧混合气体而导致的脑乳酸水平变化，时间分辨率为2.3分钟。在NMR实验期间同时记录脑电图和心电图。当给予氧气后脑乳酸恢复到缺氧前水平时，证明了缺氧反应的可逆性。这些实验证明了高分辨率1H NMR在实时监测脑代谢病理生理学方面的适用性。
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