Olsen P S, Nexø E
Scand J Gastroenterol. 1983 Sep;18(6):771-6. doi: 10.3109/00365528309182093.
Epidermal growth factor (EGF) was purified from the rat submandibular gland to establish a radioimmunoassay. Detectable amounts of EGF were found in the rat duodenum (medium, 0.55 pmol/duodenum; range, 0.38-0.68 pmol/duodenum; no. = 10) and duodenal juice (median, 215 pmol/l; range, 95-468 pmol/l; no. = 10), whereas no EGF was detected in the jejunal extracts. Submandibular and duodenal EGF each consisted of two isopeptides (pI 4.8, 5.4, and 5.5, 6.0 respectively). The peptides behaved alike both with regard to immunoreactivity (radioimmunoassay) and receptor binding (radioreceptor assay). The present data combined with previous results suggest a role for EGF in the gastrointestinal tract.
从大鼠颌下腺中纯化表皮生长因子（EGF）以建立放射免疫测定法。在大鼠十二指肠（平均值为0.55 pmol/十二指肠；范围为0.38 - 0.68 pmol/十二指肠；n = 10）和十二指肠液（中位数为215 pmol/l；范围为95 - 468 pmol/l；n = 10）中发现了可检测量的EGF，而在空肠提取物中未检测到EGF。颌下腺和十二指肠的EGF均由两种异肽组成（分别为pI 4.8、5.4和5.5、6.0）。这些肽在免疫反应性（放射免疫测定）和受体结合（放射受体测定）方面表现相似。目前的数据与先前的结果相结合表明EGF在胃肠道中发挥作用。
