Cell-mediated immunity in subjects immunized with HLA antigens.

Production of cellular immune reaction in subjects immunized against HLA antigens was studied by the capillary migration inhibition test in vitro and by the skin test in vivo. The majority of the immunized subjects (10 out of 13) developed a cell-mediated immunity to the donor antigens. Production of HLA antibodies was demonstrable in a smaller proportion of the cases. The complementary nature of humoral and cellular responsiveness was borne out by the present observations. The result of the skin test performed simultaneously with the migration inhibition test correlated well with the result in vitro.