Kim S H, Handa H, Ishikawa M, Hirai O, Yoshida S
No To Shinkei. 1984 Feb;36(2):167-74.
Ischemic change of cerebral energy metabolism and catecholamine have already been discussed largely using biochemical quantitative assay. However, regional change and their correlation are not well understood. In the present study, the ischemic regional change of cerebral energy metabolism and catecholamine were investigated in gerbils and histochemical method. Adult either sex mongolian gerbils, weighing 50-100 g, were anesthetized with ether and the left carotid artery was ligated. After observation of clinical symptoms, the brain was frozen in situ by pouring liquid N2 after 30 min and 60 min of ischemic insult. The frozen brain was sectioned with precooled saw in the coronal plane. The brain section were placed in liquid N2 bath and illuminated with 366 nm light (UV) from a 200 watt mercury lamp and Corning filter 5840. NADH fluorescence was recorded photographically through Corning filter 3387 and 5562. Also UV reflectance was recorded through Corning filter 5840 to observe quenching effect of hemoglobin. Regional change of catecholamine was observed in the same frozen brain processed with Falck-Hillarp method. According to neurological abnormalities following left carotid ligation, animals were divided into three groups; symptomatic, borderline and asymptomatic. The intensity and distribution of tissue NADH fluorescence were closely correlated to the clinical symptoms. In the symptomatic group, both in 30 min and 60 min of ischemia, homogeneously and markedly increased fluorescence was observed in the ipsilateral temporal cortex, caudate nucleus, hypothalamus and dorsolateral thalamus. Columnar mild increase of NADH fluorescence was seen in the ipsilateral parietal cortex.(ABSTRACT TRUNCATED AT 250 WORDS)
脑能量代谢和儿茶酚胺的缺血性变化已通过生化定量分析进行了大量讨论。然而，区域变化及其相关性尚未得到很好的理解。在本研究中，采用组织化学方法在沙鼠中研究了脑能量代谢和儿茶酚胺的缺血区域变化。选用体重50 - 100克的成年蒙古沙鼠，雌雄不限，用乙醚麻醉后结扎左颈动脉。观察临床症状后，在缺血损伤30分钟和60分钟后，通过灌注液氮将脑原位冷冻。用预冷的锯将冷冻的脑在冠状平面切片。将脑切片置于液氮浴中，用200瓦汞灯发出的366纳米光（紫外线）和康宁滤光片5840照射。通过康宁滤光片3387和5562以摄影方式记录NADH荧光。同时通过康宁滤光片5840记录紫外线反射率以观察血红蛋白的淬灭效应。用Falck - Hillarp方法在相同的冷冻脑上观察儿茶酚胺的区域变化。根据左颈动脉结扎后的神经功能异常，将动物分为三组：有症状组、临界组和无症状组。组织NADH荧光的强度和分布与临床症状密切相关。在有症状组中，缺血30分钟和60分钟时，同侧颞叶皮质、尾状核、下丘脑和背外侧丘脑均观察到均匀且明显增强的荧光。同侧顶叶皮质可见柱状轻度增强的NADH荧光。（摘要截短于250字）
