Solheid C, Lauro G, Palladini G
J Neurol Sci. 1976 Nov;30(1):55-64. doi: 10.1016/0022-510x(76)90255-0.
Sera from patients with malignant and benign gliomas, as well as sera from healthy donors, were tested by indirect immunofluorescence to detect antibodies against antigens on the membrane of glioblastoma, astrocytoma, reactive perimetastatic glia, normal glia and fibroblasts in tissue culture. Sera from glioblastoma patients reacted with glioblastoma, astrocytoma and reactive glial cells; they were negative on normal glia and on fibroblasts, whereas sera from astrocytoma patients were unreactive. Sera from control patients were positive in 7 out of 15 cases, although some differences were noted in the pattern of reaction. Absorption with astrocytoma powder, with glioblastoma and reactive glial cells indicated that all the positive cell lines expressed an astrocytoma-associated antigen "A", while only glioblastoma lines and reactive glial line shared a supplementary antigen "G". Neither of these 2 antigens seemed to be present in significant amount in normal brain, since the positive reactions could not be abolished by absorption with normal brain powder. The relationship between these 2 antigens and the process of increasing malignancy in gliomas is briefly discussed.
采用间接免疫荧光法检测恶性和良性神经胶质瘤患者以及健康供者血清中针对胶质母细胞瘤、星形细胞瘤、转移性周围反应性胶质细胞、正常胶质细胞和组织培养中的成纤维细胞膜上抗原的抗体。胶质母细胞瘤患者的血清与胶质母细胞瘤、星形细胞瘤和反应性胶质细胞发生反应；与正常胶质细胞和成纤维细胞呈阴性反应，而星形细胞瘤患者的血清无反应。对照患者的血清在15例中有7例呈阳性，尽管在反应模式上存在一些差异。用星形细胞瘤粉末、胶质母细胞瘤和反应性胶质细胞进行吸收试验表明，所有阳性细胞系均表达一种星形细胞瘤相关抗原“A”，而只有胶质母细胞瘤系和反应性胶质细胞系共享一种补充抗原“G”。这两种抗原在正常脑组织中似乎都不存在大量表达，因为用正常脑粉末吸收不能消除阳性反应。本文简要讨论了这两种抗原与神经胶质瘤恶性程度增加过程之间的关系。
