Carrel S, de Tribolet N, Mach J P
Acta Neuropathol. 1982;57(2-3):158-64. doi: 10.1007/BF00685384.
The reactivity spectrum of five different monoclonal anti-melanoma antibodies cross-reacting with gliomas and neuroblastomas and one monoclonal anti-glioma antibody cross-reacting with melanomas and neuroblastomas was investigated. Comparison of the binding activity of these monoclonal antibodies for 11 melanoma, seven glioma, and three neuroblastoma cell lines showed that each of these clones had a different pattern of cross-reactivity. The results indicated that the antigenic determinants detected by these antibodies were not associated with the same antigen and thus suggested the existence of at least six different antigens common to melanomas, gliomas, and neuroblastomas. Since all these tumors are known to derive from cells originating embryologically from the neural crest, it can be assumed that the antigens recognized by our monoclonal antibodies are neuroectodermal differentiation antigens. However, absorption with fetal brain homogenates abolished only the binding of monoclonal anti-glioma antibody, but did not modify the binding of monoclonal anti-melanoma antibodies.
研究了五种与神经胶质瘤和神经母细胞瘤发生交叉反应的不同单克隆抗黑色素瘤抗体以及一种与黑色素瘤和神经母细胞瘤发生交叉反应的单克隆抗神经胶质瘤抗体的反应谱。比较这些单克隆抗体对11种黑色素瘤、7种神经胶质瘤和3种神经母细胞瘤细胞系的结合活性，结果显示这些克隆中的每一个都有不同的交叉反应模式。结果表明，这些抗体检测到的抗原决定簇与同一抗原无关，因此提示黑色素瘤、神经胶质瘤和神经母细胞瘤至少存在六种不同的共同抗原。由于已知所有这些肿瘤均起源于胚胎学上源自神经嵴的细胞，因此可以假定我们的单克隆抗体识别的抗原是神经外胚层分化抗原。然而，用胎脑匀浆吸收仅消除了单克隆抗神经胶质瘤抗体的结合，但并未改变单克隆抗黑色素瘤抗体的结合。
