Antonov V F, Petrov V V, Molnar A A, Predvoditelev D A, Ivanov A S
Nature. 1980 Feb 7;283(5747):585-6. doi: 10.1038/283585a0.
Ion-specific channels in artificial membranes have been formed by the addition of gramicidin A, alamethicin, polyene antibiotics and some proteins to the solution surrounding the bilayer lipid membrane. Until now there have been no reports of single-ion channels in unmodified lipid membranes. We have now studied the electrical conductance of planar lipid bilayers membranes made of synthetic distearoylphosphorylcholine (DSPC). Current fluctuations of amplitude approximately 1pA and duration approximately 1 s have been discovered at phase transition temperature, which shows that the appearance of ionic channels may be the result of lipid domain interactions. This would explain the dramatic increase in ion permeability observed in liposomes during phase transition. We suggest that these channels could conduct the transmembrane ionic current in biological membranes without the involvement of peptides and proteins.
通过向双层脂质膜周围的溶液中添加短杆菌肽A、阿拉霉素、多烯抗生素和一些蛋白质，已在人工膜中形成了离子特异性通道。到目前为止，尚无关于未修饰脂质膜中单一离子通道的报道。我们现在研究了由合成二硬脂酰磷脂酰胆碱（DSPC）制成的平面脂质双层膜的电导。在相变温度下发现了幅度约为1pA、持续时间约为1秒的电流波动，这表明离子通道的出现可能是脂质域相互作用的结果。这可以解释在相变过程中脂质体中观察到的离子通透性的急剧增加。我们认为这些通道可以在不涉及肽和蛋白质的情况下传导生物膜中的跨膜离子电流。
