Tverdynin M S, Bulanova T P, Chernysheva E S
Arkh Patol. 1980;42(1):80-1.
A new method of azure-eosin staining of histological preparations during fixation with a 10% formalin solution, as well as of the cartilaginous and medullar tissues after decalcification in the 10% trilon B solution, pH 7,3 or in the de Castro liquid is described. This method has a number of advantages over the method os hematoxylin-eosin staining: it presents not only a clear idea about all cellular elements but also allows one to stain mast cells, fungi, microbes, Rickettsia, mucus, fibrin, necrobiotic changes in cells. Differentiation of hemopoietic cells and elements of reticuloendothelium, histiocytes is possible in the hemopoietic tissue. The method is accessible and simple and does not require special equipment.
本文描述了一种新的组织学标本苏木精 - 伊红染色方法,该方法用于在10%福尔马林溶液固定过程中,以及在pH值为7.3的10%氨三乙酸(Trilon B)溶液或德卡斯特罗液中脱钙后的软骨组织和骨髓组织染色。与苏木精 - 伊红染色方法相比,该方法具有许多优点:它不仅能清晰地显示所有细胞成分,还能对肥大细胞、真菌、微生物、立克次氏体、黏液、纤维蛋白、细胞坏死变化进行染色。在造血组织中,可以区分造血细胞、网状内皮系统成分和组织细胞。该方法易于操作且简单,不需要特殊设备。