El Kholy A M, Hafez K, Guirguis N, Facklam R R, Johnson D R, Wannamaker L
Infect Immun. 1980 Mar;27(3):953-9. doi: 10.1128/iai.27.3.953-959.1980.
Extracting type 6 group A streptococcai with dilute nitrous acid releases an antigen that exhibits type-specific precipitating properties with anti-M6 serum but fails to neutralize the type-specific bactericidal reaction or to absorb the bactericidal antibody from anti-M6 serum. These studies suggest that the precipitating and antiphagocytic properties of the M protein are attributable to different antigenic determinants. The antigenic determinant responsible for bactericidal antibody is more sensitive to the deleterious effect of nitrous acid than is the moiety responsible for the precipitin reaction. Use of the indirect fluorescent technique with anti-M sera absorbed with lyophilized nitrous acid extracts permits the possible differentiation of the two M-protein determinants in situ on the surface of streptococcal cells.
用稀亚硝酸提取6型A群链球菌会释放出一种抗原，该抗原与抗M6血清呈现型特异性沉淀特性，但不能中和型特异性杀菌反应，也不能从抗M6血清中吸收杀菌抗体。这些研究表明，M蛋白的沉淀和抗吞噬特性归因于不同的抗原决定簇。负责杀菌抗体的抗原决定簇比亚硝酸对沉淀反应负责的部分对亚硝酸的有害作用更敏感。使用用冻干亚硝酸提取物吸收的抗M血清的间接荧光技术，可以在链球菌细胞表面原位区分两种M蛋白决定簇。
