用非离子去污剂提取的链球菌M蛋白。II. 对M蛋白分子多种抗原决定簇的抗体反应分析。
Streptococcal M protein extracted by nonionic detergent. II. Analysis of the antibody response to the multiple antigenic determinants of the M-protein molecule.
作者信息
Fischetti V A
出版信息
J Exp Med. 1977 Oct 1;146(4):1108-23. doi: 10.1084/jem.146.4.1108.
Abstract
Purified streptococcal M protein extracted by nonionic detergent was used in an RIA and a solid-phase radiocompetitive inhibition assay to determine the nature of the immune response in both human beings and hyperimmunized rabbits to this complex antiphagocytic antigen. Results indicate that a type-specific response to an M antigen with the development of opsonic antibodies is the result of antibodies directed against the majority of the antigenic determinants of the molecule. Cross-reactions between certain M types on the other hand, are represented by antibodies directed against only a small percentage of these antigenic determinants. Results also suggest that avidity may play a role in the action of opsonic antibodies. However, the data indicate that factors besides avidity (i.e. sites bound by the antibodies) also seem essential for opsonization.
摘要
用非离子去污剂提取的纯化链球菌M蛋白用于放射免疫分析和固相放射竞争性抑制分析，以确定人类和经高度免疫的兔对这种复杂抗吞噬抗原的免疫反应性质。结果表明，针对M抗原产生调理素抗体的型特异性反应是针对该分子大多数抗原决定簇的抗体所致。另一方面，某些M型之间的交叉反应表现为仅针对这些抗原决定簇小部分的抗体。结果还表明亲和力可能在调理素抗体的作用中发挥作用。然而，数据表明除亲和力（即抗体结合位点）外的其他因素对于调理作用似乎也必不可少。
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Streptococcal M protein extracted by nonionic detergent. II. Analysis of the antibody response to the multiple antigenic determinants of the M-protein molecule.用非离子去污剂提取的链球菌M蛋白。II. 对M蛋白分子多种抗原决定簇的抗体反应分析。
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