• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Morphology of Borrelia burgdorferi: structural patterns of cultured borreliae in relation to staining methods.

作者信息

Aberer E, Duray P H

机构信息

Second Department of Dermatology, University of Vienna, Austria.

出版信息

J Clin Microbiol. 1991 Apr;29(4):764-72. doi: 10.1128/jcm.29.4.764-772.1991.

DOI:10.1128/jcm.29.4.764-772.1991
PMID:1716264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269867/
Abstract

The microscopic recognition of Borrelia burgdorferi in biologic fluids and tissues is difficult and challenging because of low numbers of organisms occurring as single isolated spirochetes, the apparent lack of colony formation in tissues, and differing lengths and structural morphologies. To identify the most common morphologic forms, we studied numerous cultures by a variety of microscopic techniques. Culture suspensions of B. burgdorferi were stained by several different histochemical procedures (Gram, Wright, Wright-Giemsa, Giemsa, and polychromes), fluorochromes (thioflavin-T, acridine orange, and rhodamine), silver impregnation techniques (Warthin-Starry, modified Dieterle, modified microwave Dieterle, and Bosma-Steiner) and immunocytochemical methods with different polyclonal and monoclonal antibodies. Additionally, borrelia culture suspensions were embedded in agar and also injected into skin biopsies and processed for histologic study. The different staining properties were found to be influenced by fixation methods and the type of antibody in immunocytochemical stains. This study provides evidence for marked cytomorphic variations in individual spirochetes. Variations detected by these staining procedures provide a basis for future study of tissue sections and for how borreliae can be expected to appear in tissue sections.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/0acacd227a1b/jcm00040-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/40b7470dcf89/jcm00040-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/53b43eba513e/jcm00040-0117-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/58f8cb4280e9/jcm00040-0117-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/7bc78c30c318/jcm00040-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/3a1c236cc782/jcm00040-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/d5dfd08bc7c3/jcm00040-0119-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/586ed59848a1/jcm00040-0119-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/09b264e357ec/jcm00040-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/5117f8c54e0e/jcm00040-0120-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/6d1228832a59/jcm00040-0120-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/0acacd227a1b/jcm00040-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/40b7470dcf89/jcm00040-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/53b43eba513e/jcm00040-0117-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/58f8cb4280e9/jcm00040-0117-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/7bc78c30c318/jcm00040-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/3a1c236cc782/jcm00040-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/d5dfd08bc7c3/jcm00040-0119-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/586ed59848a1/jcm00040-0119-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/09b264e357ec/jcm00040-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/5117f8c54e0e/jcm00040-0120-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/6d1228832a59/jcm00040-0120-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39db/269867/0acacd227a1b/jcm00040-0121-a.jpg

相似文献

1
Morphology of Borrelia burgdorferi: structural patterns of cultured borreliae in relation to staining methods.
J Clin Microbiol. 1991 Apr;29(4):764-72. doi: 10.1128/jcm.29.4.764-772.1991.
2
Heterogeneity of Borrelia burgdorferi in the skin.皮肤中伯氏疏螺旋体的异质性。
Am J Dermatopathol. 1996 Dec;18(6):571-9. doi: 10.1097/00000372-199612000-00004.
3
Phenotypic analysis of outer surface protein C (OspC) of Borrelia burgdorferi sensu lato by monoclonal antibodies: relationship to genospecies and OspA serotype.用单克隆抗体对伯氏疏螺旋体复合群外表面蛋白C（OspC）进行表型分析：与基因种和OspA血清型的关系
J Clin Microbiol. 1995 Jan;33(1):103-9. doi: 10.1128/jcm.33.1.103-109.1995.
4
Visualization of Borrelia burgdorferi sensu lato by fluorescence in situ hybridization (FISH) on whole-body sections of Ixodes ricinus ticks and gerbil skin biopsies.通过荧光原位杂交（FISH）对蓖麻硬蜱全身切片和沙鼠皮肤活检组织进行伯氏疏螺旋体狭义种的可视化观察。
Microbiology (Reading). 2001 Jun;147(Pt 6):1425-1436. doi: 10.1099/00221287-147-6-1425.
5
In vitro inhibition of Borrelia burgdorferi growth by antibodies.抗体对伯氏疏螺旋体生长的体外抑制作用。
J Infect Dis. 1993 Jan;167(1):165-72. doi: 10.1093/infdis/167.1.165.
6
Response of the meadow vole (Microtus pennsylvanicus) to experimental inoculation with Borrelia burgdorferi.
J Wildl Dis. 1994 Jul;30(3):408-16. doi: 10.7589/0090-3558-30.3.408.
7
Killing of Borrelia burgdorferi by macrophages is dependent on oxygen radicals and nitric oxide and can be enhanced by antibodies to outer surface proteins of the spirochete.巨噬细胞对伯氏疏螺旋体的杀伤作用依赖于氧自由基和一氧化氮，并且可被针对该螺旋体外膜蛋白的抗体增强。
Immunol Lett. 1994 May;40(2):139-46. doi: 10.1016/0165-2478(94)90185-6.
8
Identification of Borrelia burgdorferi in diffuse fasciitis with peripheral eosinophilia: borrelial fasciitis.
JAMA. 1994 Oct 26;272(16):1283-5.
9
Detection of borreliacidal antibody by using acridine orange and flow cytometry.使用吖啶橙和流式细胞术检测杀博氏疏螺旋体抗体。
Clin Diagn Lab Immunol. 1994 Jan;1(1):44-50. doi: 10.1128/cdli.1.1.44-50.1994.
10
Borrelia burgdorferi induces the production and release of proinflammatory cytokines in canine synovial explant cultures.伯氏疏螺旋体在犬滑膜外植体培养物中诱导促炎细胞因子的产生和释放。
Infect Immun. 1998 Jan;66(1):247-58. doi: 10.1128/IAI.66.1.247-258.1998.

引用本文的文献

1
Presence of Borrelia Spirochetes in White Stork (), White-Tailed Eagle (), and Eastern Imperial Eagle (): Hospitalized in a Wild Bird Hospital and Sanctuary (Hortobágy, Hungary).白鹳（）、白尾海雕（）和东方白背兀鹫（）体内存在伯氏疏螺旋体：在野生鸟类医院及保护区（匈牙利霍托巴吉）接受治疗。
Animals (Basel). 2024 Dec 10;14(24):3553. doi: 10.3390/ani14243553.
2
and Exhibit Antioxidant and Inhibiting Activities.并表现出抗氧化和抑制活性。
Int J Mol Sci. 2024 Jun 28;25(13):7112. doi: 10.3390/ijms25137112.
3
Is There a Role for Dark Field Microscopy in the Diagnosis of Lyme Disease?A Narrative Review.

本文引用的文献

1
Lyme disease-a tick-borne spirochetosis?莱姆病——一种蜱传播的螺旋体病？
Science. 1982 Jun 18;216(4552):1317-9. doi: 10.1126/science.7043737.
2
The spirochetal etiology of Lyme disease.莱姆病的螺旋体病因
N Engl J Med. 1983 Mar 31;308(13):733-40. doi: 10.1056/NEJM198303313081301.
3
Repeated isolation of spirochetes from the cerebrospinal fluid of a patient with meningoradiculitis Bannwarth.从一名患有班恩瓦尔特脑脊神经根炎患者的脑脊液中反复分离出螺旋体。
暗视野显微镜在莱姆病诊断中是否有作用？一项叙述性综述。
Infect Dis Clin Microbiol. 2023 Dec 29;5(4):281-286. doi: 10.36519/idcm.2023.291. eCollection 2023 Dec.
4
A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood.一种便携式免疫传感器可灵敏、快速地检测血样中的伯氏疏螺旋体抗原。
Sci Rep. 2023 May 9;13(1):7546. doi: 10.1038/s41598-023-34108-9.
5
A Comparative Study of Body Lice and Bed Bugs Reveals Factors Potentially Involved in Differential Vector Competence for the Relapsing Fever Spirochete Borrelia recurrentis.体虱和臭虫的比较研究揭示了与回归热螺旋体伯氏疏螺旋体的差异传播能力相关的潜在因素。
Infect Immun. 2022 May 19;90(5):e0068321. doi: 10.1128/iai.00683-21. Epub 2022 Apr 6.
6
Molecular Mechanisms of   Phagocytosis and Intracellular Processing by Human Macrophages.人类巨噬细胞吞噬作用及细胞内加工处理的分子机制
Biology (Basel). 2021 Jun 22;10(7):567. doi: 10.3390/biology10070567.
7
Laboratory Diagnosis of Lyme Borreliosis.莱姆病的实验室诊断。
Clin Microbiol Rev. 2021 Jan 27;34(2). doi: 10.1128/CMR.00018-19. Print 2021 Mar 17.
8
SNX3 drives maturation of phagosomes by forming a hub for PI(3)P, Rab5a, and galectin-9.SNX3 通过形成一个包含 PI(3)P、Rab5a 和半乳糖凝集素-9 的中心来驱动吞噬体的成熟。
J Cell Biol. 2019 Sep 2;218(9):3039-3059. doi: 10.1083/jcb.201812106. Epub 2019 Jul 23.
9
Outer surface protein OspC is an antiphagocytic factor that protects Borrelia burgdorferi from phagocytosis by macrophages.外表面蛋白OspC是一种抗吞噬因子，可保护伯氏疏螺旋体免受巨噬细胞的吞噬作用。
Infect Immun. 2015 Dec;83(12):4848-60. doi: 10.1128/IAI.01215-15. Epub 2015 Oct 5.
10
Morphological and biochemical features of Borrelia burgdorferi pleomorphic forms.伯氏疏螺旋体多形形态的形态学和生化特征
Microbiology (Reading). 2015 Mar;161(Pt 3):516-27. doi: 10.1099/mic.0.000027. Epub 2015 Jan 6.
Eur J Clin Microbiol. 1984 Dec;3(6):564-5. doi: 10.1007/BF02013623.
4
Isolation and cultivation of Lyme disease spirochetes.莱姆病螺旋体的分离与培养。
Yale J Biol Med. 1984 Jul-Aug;57(4):521-5.
5
A modified Warthin-Starry procedure at low pH for melanin.一种针对黑色素的低pH值改良Warthin-Starry染色法。
Am J Clin Pathol. 1980 Jun;73(6):812-5. doi: 10.1093/ajcp/73.6.812.
6
Successful cultivation of spirochetes from skin lesions of patients with erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans.从慢性游走性红斑患者阿费利乌斯皮肤损伤处及萎缩性慢性肢端皮炎中成功培养出螺旋体。
Acta Pathol Microbiol Immunol Scand B. 1985 Apr;93(2):161-3. doi: 10.1111/j.1699-0463.1985.tb02870.x.
7
Histological demonstration of spirochaetes: a method of preparing control sections.
Med Lab Sci. 1985 Jul;42(3):283-4.
8
Colony formation and morphology in Borrelia burgdorferi.伯氏疏螺旋体的菌落形成与形态
J Clin Microbiol. 1987 Nov;25(11):2054-8. doi: 10.1128/jcm.25.11.2054-2058.1987.
9
European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility.从人类和蜱虫中分离出的欧洲伯氏疏螺旋体的培养条件和抗生素敏感性。
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):112-8. doi: 10.1016/s0176-6724(86)80110-9.
10
Biology of Borrelia species.疏螺旋体属的生物学
Microbiol Rev. 1986 Dec;50(4):381-400. doi: 10.1128/mr.50.4.381-400.1986.