通过抑制多形核白细胞化学发光检测人血清中的免疫复合物。
Inhibition of polymorphonuclear leukocyte chemiluminescence for detection of immune complexes in human sera.
作者信息
Doll N J, Wilson M R, Salvaggio J E
出版信息
J Clin Invest. 1980 Sep;66(3):457-64. doi: 10.1172/JCI109876.
An assay for the detection and quantitation of immune complexes is described. Experimental immune complexes or aggregated human gamma globulin (AHG) were incubated with polymorphonuclear leukocytes (PMN). After challenge of the PMN with opsonized zymosan, chemiluminescence was recorded in a scintillation spectrometer. A quantitative inhibition of chemiluminescence could be demonstrated by the interaction of PMN with immune complexes or AHG. Experimental immune complexes of bovine serum albumin-anti-bovine serum albumin were formed and tested by this assay, and immune complexes formed near antigen excess were best described by this technique. The technique was used to demonstrate immune complexes in the sera from patients with systemic lupus erythematosus, rheumatoid arthritis, and vasculitis. Immune complexes were quantitated by reference to a standard curve using AHG. By this technique, normal human sera had < 10 micrograms of AHG per milliliter of serum. Immune complexes at levels above this were detected in 9/15 patients with systemic lupus erythematosus, 18/30 patients with rheumatoid arthritis, and 2/5 patients with vasculitis. Therefore, this assay is a sensitive, simple method for measurement of circulating immune complexes in the sera of patients with certain connective tissue diseases.
本文描述了一种用于检测和定量免疫复合物的分析方法。将实验性免疫复合物或聚合人γ球蛋白（AHG）与多形核白细胞（PMN）一起孵育。在用调理酵母聚糖刺激PMN后，在闪烁光谱仪中记录化学发光。PMN与免疫复合物或AHG的相互作用可证明化学发光的定量抑制。形成牛血清白蛋白-抗牛血清白蛋白的实验性免疫复合物并通过该分析方法进行测试，该技术最能描述在抗原过量附近形成的免疫复合物。该技术用于证明系统性红斑狼疮、类风湿性关节炎和血管炎患者血清中的免疫复合物。通过参考使用AHG的标准曲线对免疫复合物进行定量。通过该技术，正常人血清每毫升血清中AHG含量<10微克。在15例系统性红斑狼疮患者中的9例、30例类风湿性关节炎患者中的18例和5例血管炎患者中的2例中检测到高于该水平的免疫复合物。因此，该分析方法是一种用于测量某些结缔组织疾病患者血清中循环免疫复合物的灵敏、简单的方法。
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