Steffelaar J W, Ten Kate F J, Nap M, Swaak A J, De Graaffreitsma C B, Van Elven E H, Feltkamp-Vroom T M
Clin Exp Immunol. 1977 Mar;27(3):391-6.
Polymorphonuclear leucocytes (PMN) from patients with systemic lupus erythematosus (SLE) were isolated from defibrinated and heparinized blood. In addition, PMN from a healthy donor were incubated with sera from SLE patients and with sera containing artificially prepared immune complexes of hepatitis B surface antigen (HBsAg) and human anti-HBsAg immunoglobulin (anti-HBs) with well defined variations of the antigen/antibody ratio. To one group of blood samples, 5 mM monoiodine acetic acid (MIAA) was added to block in vitro phagocytosis. The Pmn were examined for the presence of IgG, IgM, and HBsAg by the immunofluorescence technique. PMN from defibrinated blood of SLE patients showed in up to 80% immunoglobulin (Ig)-inclusions. However, addition of 5 mM MIAA reduced the number of Ig-containing PMN to at most 40%, which levels were equal to numbers found in specimens from heparinized blood. Addition of 5 mM MIAA to heparinized blood did not reduce the number of PMN with Ig inclusions. Normal donor PMN isolated from defibrinated, heparinized, and EDT blood showed equal amounts of Ig inclusions after incubation with SLE sera, but none when MIAA had been added. In PMN incubated with HBsAg-anti HBs immune complexes with an antigen antibody ratio between 5 and 0-2, both HBsAg and IgG could be detected. It is concluded that Ig inclusions in PMN from heparinized blood from SLE patients are due to in vivo phagocytosis, presumably of circulating immune complexes. In vitro phagocytosis of Ig from SLE sera by normal donor PMN also suggests the presence of immune complexes. Dependent on the antigen-antibody ratio, artificial HBsAg/anti-HBs immune complexes can be detected by in vitro phagocytosis by PM.
从患有系统性红斑狼疮（SLE）患者的去纤维蛋白和肝素化血液中分离出多形核白细胞（PMN）。此外，将来自健康供体的PMN与SLE患者的血清以及含有乙肝表面抗原（HBsAg）和人抗-HBsAg免疫球蛋白（抗-HBs）的人工制备的免疫复合物（抗原/抗体比例有明确变化）的血清一起孵育。向一组血样中加入5 mM一碘乙酸（MIAA）以阻断体外吞噬作用。通过免疫荧光技术检测PMN中IgG、IgM和HBsAg的存在情况。来自SLE患者去纤维蛋白血液的PMN显示高达80%有免疫球蛋白（Ig）包涵体。然而，加入5 mM MIAA后，含Ig的PMN数量最多减少到40%，这一水平与来自肝素化血液标本中的数量相当。向肝素化血液中加入5 mM MIAA并未减少有Ig包涵体的PMN数量。从去纤维蛋白、肝素化和EDTA血液中分离的正常供体PMN在与SLE血清孵育后显示出等量的Ig包涵体，但加入MIAA后则没有。在与抗原抗体比例在5至0-2之间的HBsAg-抗-HBs免疫复合物孵育的PMN中，可同时检测到HBsAg和IgG。结论是，SLE患者肝素化血液中PMN的Ig包涵体是由于体内吞噬作用，可能是循环免疫复合物的吞噬作用。正常供体PMN对SLE血清中Ig的体外吞噬作用也表明存在免疫复合物。根据抗原-抗体比例，人工HBsAg/抗-HBs免疫复合物可通过PMN的体外吞噬作用检测到。
