关于博来霉素在体外或体内处理EMT6小鼠肿瘤细胞时“潜在致死性损伤”的研究。
Studies of "potentially lethal damage" in EMT6 mouse tumour cells treated with bleomycin either in vitro or in vivo.
作者信息
Twentyman P R, Bleehen N M
出版信息
Br J Cancer. 1975 Oct;32(4):491-501. doi: 10.1038/bjc.1975.251.
Abstract
Studies have been carried out into the effect usually referred to as "repair to potentially lethal damage" following the treatment of cells with bleomycin. In vitro, increased survival was seen with delayed subculture of cells in both exponential phase and plateau phase. It was unimportant whether the medium present during the delay period had been previously used to support cell growth. Exposure of cells growing as a solid tumour in vivo to bleomycin (4 mg/kg), gave a surviving fraction of 2 X 10(-3) if assay was carried out at 30 min but a surviving fraction of virtually 100% if assay was delayed until 6 h. Various possible artefacts have been eliminated as reasons for the observations but doubts are raised regarding the nature of the mechanism involved.
摘要
针对博来霉素处理细胞后通常被称为“对潜在致死性损伤的修复”的效应，已经开展了多项研究。在体外，处于指数生长期和平台期的细胞进行延迟传代培养时，细胞存活率均有所提高。延迟期所用的培养基之前是否用于支持细胞生长并不重要。体内实体瘤生长的细胞暴露于博来霉素（4毫克/千克）后，如果在30分钟时进行检测，存活分数为2×10⁻³，但如果检测延迟至6小时，存活分数几乎为100%。已经排除了各种可能导致这些观察结果的假象，但对于所涉及机制的性质仍存在疑问。
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1
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Science. 1969 Aug 1;165(3892):490-2. doi: 10.1126/science.165.3892.490.
2
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Int J Radiat Biol Relat Stud Phys Chem Med. 1971;20(1):87-92. doi: 10.1080/09553007114550921.
3
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Radiology. 1973 Mar;106(3):689-94. doi: 10.1148/106.3.689.
4
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