Peters G G, Hu J
J Virol. 1980 Dec;36(3):692-700. doi: 10.1128/JVI.36.3.692-700.1980.
Mutants of avian sarcoma virus which lack a functional DNA polymerase were found to be nonselective in the incorporation of host cell tRNA's into virus particles. In contrast, mutants which possess a functional DNA polymerase but lack the viral genome RNA contained a specific subset of the host cell tRNA population, indistinguishable from that of the wild-type virus. Thus the reverse transcriptase, and not the viral RNA, is probably the major factor determining which tRNA's are incorporated into avian sarcoma virus particles. Supporting evidence was obtained in an in vitro binding assay between purified reverse transcriptase and unfractionated cellular tRNA's. However, the subset of tRNA's which associated with the genome in the 70S complex was determined primarily by the viral RNA. In the absence of DNA polymerase, the 70S RNA complex in mature virus particles contained the normal complement of associated tRNA's with the exception of tRNATrp, the primer for RNA-directed DNA synthesis.
人们发现，缺乏功能性DNA聚合酶的禽肉瘤病毒突变体在将宿主细胞tRNA掺入病毒颗粒的过程中没有选择性。相比之下，拥有功能性DNA聚合酶但缺乏病毒基因组RNA的突变体含有宿主细胞tRNA群体中的一个特定子集，与野生型病毒的该子集无法区分。因此，逆转录酶而非病毒RNA可能是决定哪些tRNA被掺入禽肉瘤病毒颗粒的主要因素。在纯化的逆转录酶与未分级的细胞tRNA之间的体外结合试验中获得了支持证据。然而，在70S复合物中与基因组相关的tRNA子集主要由病毒RNA决定。在没有DNA聚合酶的情况下，成熟病毒颗粒中的70S RNA复合物除了作为RNA指导的DNA合成引物的tRNATrp外，含有正常互补的相关tRNA。
