Abbas A K, Burakoff S J, Gefter M L, Greene M I
J Exp Med. 1980 Oct 1;152(4):969-78. doi: 10.1084/jem.152.4.969.
To investigate the mechanisms by which T lymphocytes regulate myeloma function in vitro, the effects of regulatory T cells on antibody secretion by a hybrid myeloma cell line were examined. Suppressor T cells (Ts) specific for idiotypic determinants on M315 (IgA, lambda 2 anti-2,4-dinitrophenol and anti-2,4,6-trinitrophenol [TNP]) and MPC 11 (IgG2b, kappa) myeloma proteins inhibit antibody secretion by the appropriate parental myeloma cells. When cocultured with a hybrid cell line derived by fusion of MOPC 315 and MPC 11 myelomas, the idiotype-reactive Ts inhibit secretion of only the immunoglobulin (Ig) bearing the relevant idiotype. In contrast, syngeneic TNP-reactive cytolytic T lymphocytes (CTL) inhibit antibody secretion by TNP-binding MOPC 315 cells but not by MPC 11 cells in the presence of soluble TNP-keyhole limpet hemocyanin (KLH), and this inhibition probably represents a prelytic effect of the CTL. Such TNP-reactive CTL, in the presence of TNP-KLH, inhibit both IgA and IgG secretion by the MOPC 315-MPC 11 hybrid, which is consistent with a prelytic effect. Thus, myeloma hybrids are a useful tool for investigating the effector function of regulatory T cells. These results are discussed with reference to the mechanisms of action of regulatory T cells and their relevance to modulation of physiologic humoral immune responses.
为了研究T淋巴细胞在体外调节骨髓瘤功能的机制，检测了调节性T细胞对杂交骨髓瘤细胞系抗体分泌的影响。针对M315（IgA，λ2抗-2,4-二硝基苯酚和抗-2,4,6-三硝基苯酚[TNP]）和MPC 11（IgG2b，κ）骨髓瘤蛋白独特型决定簇的抑制性T细胞（Ts）可抑制相应亲本骨髓瘤细胞的抗体分泌。当与由MOPC 315和MPC 11骨髓瘤融合产生的杂交细胞系共培养时，独特型反应性Ts仅抑制携带相关独特型的免疫球蛋白（Ig）的分泌。相比之下，在可溶性TNP-钥孔戚血蓝蛋白（KLH）存在的情况下，同基因TNP反应性细胞毒性T淋巴细胞（CTL）抑制TNP结合的MOPC 315细胞的抗体分泌，但不抑制MPC 11细胞的抗体分泌，这种抑制可能代表CTL的裂解前效应。在TNP-KLH存在的情况下，这种TNP反应性CTL抑制MOPC 315-MPC 11杂交瘤细胞的IgA和IgG分泌，这与裂解前效应一致。因此，骨髓瘤杂交瘤是研究调节性T细胞效应功能的有用工具。参考调节性T细胞的作用机制及其与生理性体液免疫反应调节的相关性对这些结果进行了讨论。
