Dickinson J P, McDermott J R, Smith J K, Caspary E A
Br J Cancer. 1980 Aug;42(2):266-74. doi: 10.1038/bjc.1980.226.
Intact cells of various human tumours and tumour cell lines, and acid extracts of various human tumours and normal tissues, each of which react with the lymphocytes of cancer patients as detected by the macrophage electrophoretic mobility (MEM) test, have been subjected to proteolysis. Activity was destroyed by some enzymes, and the apparent molecular size of the active material was reduced by others. An active low-mol.-wt fragment has been partially purified from papain digests of several tumours. Peptides with normal tissue and tumour-characteristic activities have been separated chromatographically from tryptic digests of tumour extracts.
各种人类肿瘤及其细胞系的完整细胞，以及各种人类肿瘤和正常组织的酸提取物，通过巨噬细胞电泳迁移率（MEM）试验检测，它们均能与癌症患者的淋巴细胞发生反应，现已对其进行了蛋白水解处理。某些酶会破坏活性，而其他酶则会降低活性物质的表观分子大小。已从几种肿瘤的木瓜蛋白酶消化物中部分纯化出一种活性低分子量片段。已通过色谱法从肿瘤提取物的胰蛋白酶消化物中分离出具有正常组织和肿瘤特征活性的肽。
