Minden P, Mathews H L
Cancer Res. 1980 Sep;40(9):3214-7.
Heat-killed Mycobacterium bovis strain Bacillus Calmette-Guérin cells were sonically disrupted, and their antitumor effects against the line 10 hepatocarcinoma in strain 2 guinea pigs were evaluated. When injected together with viable line 10 cells, there was complete suppression of tumor growth. Growth of tumor was also suppressed when line 10 cells were injected contralaterally at the same time as the vaccine mixture. Multiple intratumor injections of sufficient disrupted M. bovis strain B. Calmette-Guérin were therapeutically effective against 74% of 7-day-old tumors and against 40% of 14-day-old tumors. Surviving animals were usually resistant to subsequent rechallenges with line 10 cells but not to syngeneic L2C leukemia cells. By means of a competitive radioimmunoassay, antigenic determinants were detected that were expressed by disrupted but not by intact bacteria.
将热灭活的牛分枝杆菌卡介苗菌株细胞进行超声破碎，并评估其对2系豚鼠10号肝癌细胞系的抗肿瘤作用。当与活的10号细胞系一起注射时，肿瘤生长被完全抑制。当在注射疫苗混合物的同时在对侧注射10号细胞系时，肿瘤生长也受到抑制。对7日龄肿瘤，多次瘤内注射足够量的破碎牛分枝杆菌卡介苗菌株有治疗效果，对14日龄肿瘤的治疗有效率为40%。存活的动物通常对随后再次接种10号细胞系有抗性，但对同基因L2C白血病细胞没有抗性。通过竞争性放射免疫测定法，检测到了由破碎细菌而非完整细菌表达的抗原决定簇。
