Yarkoni E, Ashley M P, Zbar B, Sugimoto T, Rapp H J
Cancer Res. 1982 Jul;42(7):2544-6.
Guinea pigs, each with an established syngeneic dermal line 10 tumor and microscopic lymph node metastases, were immunized by injection of a mixture of irradiated line 10 tumor cells and an oil-in-water emulsion containing heat-killed cells of Mycobacterium bovis strain Bacillus Calmette-Guérin. Squalane or squalene-in-water emulsions, prepared by ultrasonication and containing mg doses of mycobacterial cells, were effective adjuvants. Immunization eradicated established dermal tumors (about 10 mm in diameter) and prevented growth of microscopic lymph node metastases. Untreated animals, animals treated by intradermal administration of Bacillus Calmette-Guérin cells attached to oil droplets alone or with irradiated tumor cells alone, all died with progressive tumor growth.
豚鼠每只都已建立同基因10号线皮肤肿瘤并有微小的淋巴结转移，通过注射经辐照的10号线肿瘤细胞与含有热灭活卡介苗（Bacillus Calmette - Guérin）牛分枝杆菌细胞的水包油乳剂的混合物进行免疫。通过超声处理制备的含有毫克剂量分枝杆菌细胞的角鲨烷或水包角鲨烯乳剂是有效的佐剂。免疫消除了已形成的皮肤肿瘤（直径约10毫米）并阻止了微小淋巴结转移的生长。未治疗的动物、仅通过皮内注射附着在油滴上的卡介苗细胞或仅与经辐照的肿瘤细胞一起治疗的动物，均因肿瘤进行性生长而死亡。
