Bussolino F, Benveniste J
Immunology. 1980 Jul;40(3):367-76.
Basophil-rich rabbit leucocytes sensitized by anti-horseradish peroxidase antibodies released platelet-activating factor (PAF) and histamine upon exposure to the specific antigen. This release was preceded and accompanied by a sharp decrease in the intracellular concentration of cyclic AMP. Isoproterenol, a beta-adrenergic agent, and theophylline, a phosphodiesterase inhibitor, used individually or in combination, increased the intracellular concentration of cyclic AMP and inhibited the release of both PAF and histamine. Propranolol, a beta-adrenergic blocking agent, suppressed the effect of isoproterenol on cyclic AMP level and mediator release. Dibutyryl cyclic AMP, an alkylated derivative of cyclic AMP, inhibited PAF and histamine release. These results indicate that cyclic AMP, which is known to control the release of other mediators of immediate hypersensitivity, also regulates the release of PAF. Histamine and PAF followed one another closely in all of our release or inhibition experiments, bringing more evidence for the basophil origin of PAF.
用抗辣根过氧化物酶抗体致敏的富含嗜碱性粒细胞的兔白细胞，在接触特异性抗原后会释放血小板活化因子（PAF）和组胺。在这种释放之前并伴随有细胞内环磷酸腺苷（cAMP）浓度的急剧下降。异丙肾上腺素（一种β-肾上腺素能剂）和氨茶碱（一种磷酸二酯酶抑制剂）单独或联合使用时，会增加细胞内cAMP的浓度，并抑制PAF和组胺的释放。普萘洛尔（一种β-肾上腺素能阻断剂）可抑制异丙肾上腺素对cAMP水平和介质释放的作用。二丁酰环磷酸腺苷（cAMP的一种烷基化衍生物）可抑制PAF和组胺的释放。这些结果表明，已知可控制其他速发型超敏反应介质释放的cAMP，也调节PAF的释放。在我们所有的释放或抑制实验中，组胺和PAF的变化密切相关，这为PAF来源于嗜碱性粒细胞提供了更多证据。
