通过细胞内注射荧光示踪剂进行细胞谱系分析。
Cell lineage analysis by intracellular injection of fluorescent tracers.
作者信息
Weisblat D A, Zackson S L, Blair S S, Young J D
出版信息
Science. 1980 Sep 26;209(4464):1538-41. doi: 10.1126/science.6159680.
PMID:6159680
Abstract
Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.
摘要
通过将荧光肽罗丹明-D-肽注射到已鉴定的胚胎细胞中，揭示了水蛭发育过程中的细胞谱系。将这种肽与核染色剂一起使用，显示了干细胞及其后代的刻板分裂模式。罗丹明-D-肽和链霉蛋白酶的联合注射证明了在注射链霉蛋白酶的端细胞中干细胞产生的停滞。
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