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[用混合血细胞吸附法检测细胞中癌病毒D诱导的抗原]

[Detection of oncornavirus D-induced antigen in cells by a mixed hemadsorption method].

作者信息

Kosiakova N P, Pavliuchenkova R P, Posevaia T A

出版信息

Vopr Virusol. 1980 May-Jun(3):351-4.

PMID:6159733
Abstract

Further study of a new oncornavirus D-induced antigen was done. Its immunological specificity and localization in cells was determined by a sensitive method of mixed hemadsorption. A continuous HEp-2 cell culture was used as the source of the new as well as virus antigen. Adsorbed specific sera to the new antigen and to oncornavirus D obtained by the previously developed method were used for the antigens detection. This method demonstrated localization of both virus antigens and oncornavirus D-induced antigens not included into the virion structure on the surface of oncornavirus D-infected cells. Mixed hemadsorption test as well as complement-fixation test gave the results identical in their specificity indicating the presence in HEp-2 cells of a new non-virion antigen.

摘要

对一种新的致瘤病毒D诱导的抗原进行了进一步研究。通过一种灵敏的混合血细胞吸附方法确定了其免疫特异性和在细胞中的定位。连续的人喉上皮癌细胞(HEp-2)培养物被用作新抗原以及病毒抗原的来源。用通过先前开发的方法获得的针对新抗原和致瘤病毒D的吸附特异性血清来检测抗原。该方法证明了病毒抗原和未包含在病毒粒子结构中的致瘤病毒D诱导的抗原在感染致瘤病毒D的细胞表面的定位。混合血细胞吸附试验以及补体结合试验给出了特异性相同的结果,表明在人喉上皮癌细胞(HEp-2)中存在一种新的非病毒粒子抗原。

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