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[小鼠成纤维细胞培养中癌病毒D诱导产生的一种新抗原]

[A new antigen induced by oncornavirus D in mouse fibroblast culture].

作者信息

Korosteleva V S, Kosiakov P N, Pavliuchenkova R P, Kosiakova N P

出版信息

Vopr Virusol. 1976 Sep-Oct(5):526-31.

PMID:795160
Abstract

It was established by the CFT and immunofluorescence that oncornavirus D inoculated into continuous mouse fibroblast cultures induced in them a new antigen differing from those of the virus and of the original non-infected cells. The antigen was shown to accumulate during cultivation of the infected cells. No antigen was induced by oncornavirus inactivated by heating or neutralized by antiviral antiserum. The physico-chemical and immunological properties of the new antigen experimentally induced in the mouse fibroblast culture are identical to those of the new antigen present in cultures of human J-96, HeLa and HEp-2 cells.

摘要

补体结合试验(CFT)和免疫荧光法证实,接种于连续传代的小鼠成纤维细胞培养物中的D型肿瘤病毒可诱导产生一种新抗原,该抗原不同于病毒抗原和原始未感染细胞的抗原。结果表明,该抗原在受感染细胞培养过程中会累积。加热灭活或用抗病毒抗血清中和的肿瘤病毒均不会诱导产生抗原。在小鼠成纤维细胞培养物中通过实验诱导产生的新抗原的物理化学和免疫学特性,与人J-96、HeLa和HEp-2细胞培养物中存在的新抗原相同。

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