Folkman J, Haudenschild C
Nature. 1980 Dec 11;288(5791):551-6. doi: 10.1038/288551a0.
Cloned capillary endothelial cells, cultured in tumour-conditioned medium, form capillary tubes. By light and electron microscopy these tubes resemble capillaries in vivo. This first demonstration of angiogenesis in vitro: (1) shows that all the information necessary to develop an entire capillary network in vitro is expressed by one cell type; (2) suggests a mechanism for lumen formation; and (3) offers a possibility of distinguishing between direct and indirect angiogenesis factors.
克隆的毛细血管内皮细胞在肿瘤条件培养基中培养时会形成毛细血管管腔。通过光学显微镜和电子显微镜观察，这些管腔在体内类似于毛细血管。体外血管生成的这一首次证明：（1）表明在体外发育整个毛细血管网络所需的所有信息都由一种细胞类型表达；（2）提示了管腔形成的机制；（3）提供了区分直接和间接血管生成因子的可能性。
