Nguyen Huu Tuan, Peirsman Arne, Tirpakova Zuzana, Mandal Kalpana, Vanlauwe Florian, Maity Surjendu, Kawakita Satoru, Khorsandi Danial, Herculano Rondinelli, Umemura Christian, Yilgor Can, Bell Remy, Hanson Adrian, Li Shaopei, Nanda Himansu Sekhar, Zhu Yangzhi, Najafabadi Alireza Hassani, Jucaud Vadim, Barros Natan, Dokmeci Mehmet Remzi, Khademhosseini Ali
Terasaki Institute for Biomedical Innovation, Los Angeles, CA 90064, USA.
Plastic, Reconstructive and Aesthetic Surgery, Ghent University Hospital, 9000 Ghent, Belgium.
Micromachines (Basel). 2023 Apr 29;14(5):978. doi: 10.3390/mi14050978.
Engineered human tissues created by three-dimensional cell culture of human cells in a hydrogel are becoming emerging model systems for cancer drug discovery and regenerative medicine. Complex functional engineered tissues can also assist in the regeneration, repair, or replacement of human tissues. However, one of the main hurdles for tissue engineering, three-dimensional cell culture, and regenerative medicine is the capability of delivering nutrients and oxygen to cells through the vasculatures. Several studies have investigated different strategies to create a functional vascular system in engineered tissues and organ-on-a-chips. Engineered vasculatures have been used for the studies of angiogenesis, vasculogenesis, as well as drug and cell transports across the endothelium. Moreover, vascular engineering allows the creation of large functional vascular conduits for regenerative medicine purposes. However, there are still many challenges in the creation of vascularized tissue constructs and their biological applications. This review will summarize the latest efforts to create vasculatures and vascularized tissues for cancer research and regenerative medicine.
通过在水凝胶中对人类细胞进行三维细胞培养而构建的工程化人体组织，正成为癌症药物研发和再生医学领域新兴的模型系统。复杂的功能性工程化组织也有助于人体组织的再生、修复或替换。然而，组织工程、三维细胞培养和再生医学的主要障碍之一，是通过脉管系统为细胞输送营养物质和氧气的能力。多项研究探讨了在工程化组织和器官芯片中创建功能性血管系统的不同策略。工程化脉管系统已被用于血管生成、血管发生以及药物和细胞跨内皮运输的研究。此外，血管工程能够创建用于再生医学目的的大型功能性血管导管。然而，在构建血管化组织构建体及其生物学应用方面仍存在许多挑战。本综述将总结为癌症研究和再生医学创建脉管系统和血管化组织的最新进展。
