Torelli G, Ferrari S, Donelli A, Cadossi R, Ferrari S, Bosi P, Torelli U
Nucleic Acids Res. 1981 Dec 21;9(24):7013-32. doi: 10.1093/nar/9.24.7013.
The abundance and complexity of total poly(A)+RNA of human normal unstimulated lymphocytes, PHA-stimulated and leukemic lymphocytes were examined using the cDNA-poly(A)+ RNA hybridization technique. It was found that in both PHA-stimulated and leukemic lymphocytes the complexity of total poly(A)+RNA is strongly reduced in respect to normal unstimulated lymphocytes. The differences is due to a small portion, by weight, of high complexity sequences, suggesting the involvement or nuclear, not necessarily transcriptional, events. Moreover the heterologous hybridizations show that: 1) in PHA-stimulated lymphocytes there is a set of highly abundant sequences which are represented in normal and leukemic lymphocytes at a very reduced rate; 2) the sequences abundant in normal unstimulated lymphocytes are less represented in leukemic lymphocytes; the converse also is true, but at a lower extent. On the basis of available data, it is suggested that the observed changes in the abundance pattern of PHA-stimulated lymphocytes may be related mainly to a difference in the half-life of the mRNAs, while those of leukemic lymphocytes require some transcriptional or post-transcriptional nuclear event.
利用cDNA - poly(A)+RNA杂交技术检测了人正常未刺激淋巴细胞、PHA刺激淋巴细胞和白血病淋巴细胞中总poly(A)+RNA的丰度和复杂性。结果发现，与正常未刺激淋巴细胞相比，PHA刺激淋巴细胞和白血病淋巴细胞中总poly(A)+RNA的复杂性均显著降低。这种差异是由于一小部分（按重量计）高复杂性序列所致，提示涉及核内事件，不一定是转录事件。此外，异源杂交显示：1）在PHA刺激的淋巴细胞中有一组高度丰富的序列，在正常淋巴细胞和白血病淋巴细胞中的表达率极低；2）正常未刺激淋巴细胞中丰富的序列在白血病淋巴细胞中的表达较少；反之亦然，但程度较低。根据现有数据，提示PHA刺激淋巴细胞中观察到的丰度模式变化可能主要与mRNA半衰期的差异有关，而白血病淋巴细胞的变化则需要一些转录或转录后核内事件。
