Wilkes P R, Birnie G D, Paul J
Nucleic Acids Res. 1979;6(6):2193-208. doi: 10.1093/nar/6.6.2193.
Nuclear and polysomal polyadenylated RNA populations of normal and 16 hour regenerating rat liver have been compared by mRNA-cDNA hybridisations and by unique DNA saturation experiments. It was found that nuclear polyadenylated RNA hybridises to 6.8% of unique DNA in both normal and 16 hour regenerating rat liver. However, cross-hybridisation experiments using cDNA have shown that 10-15% by weight of nuclear polyadenylated RNA sequences are specific to 16 hour regenerating rat-liver. Since both unique DNA and cDNA hybridisation have shown that normal and 16 hour regenerating rat-liver polysomal polyadenylated RNA populations are qualitatively very similar sequences specific to 16 hour regenerating rat-liver nuclear polyadenylated RNA are nucleus confined. Polysomal RNA sequences which were abundant in normal rat-liver have become less abundant in regenerating rat liver.
通过mRNA-cDNA杂交和独特DNA饱和实验,对正常大鼠肝脏和再生16小时的大鼠肝脏的核多聚腺苷酸化RNA群体和多核糖体多聚腺苷酸化RNA群体进行了比较。结果发现,在正常大鼠肝脏和再生16小时的大鼠肝脏中,核多聚腺苷酸化RNA与6.8%的独特DNA杂交。然而,使用cDNA的交叉杂交实验表明,核多聚腺苷酸化RNA序列重量的10%-15%对再生16小时的大鼠肝脏具有特异性。由于独特DNA和cDNA杂交均表明,正常大鼠肝脏和再生16小时的大鼠肝脏多核糖体多聚腺苷酸化RNA群体在质量上非常相似,因此再生16小时的大鼠肝脏核多聚腺苷酸化RNA特有的序列局限于细胞核。正常大鼠肝脏中丰富的多核糖体RNA序列在再生大鼠肝脏中变得不那么丰富。