Nutt J G, Mrox E A, Leeman S E, Williams A C, Engel W K, Chase T N
Neurology. 1980 Dec;30(12):1280-5. doi: 10.1212/wnl.30.12.1280.
Substance P (SP), a putative peptide neurotransmitter, was measured in human lumbar cerebrospinal fluid (CSF) by radioimmunoassay. Substance P-like immunoreactivity (SPLI) was present in the CSF of 18 neurologically normal adults in concentrations ranging from 2.9 to 11.1 fmol per milliliter, with a mean of 7.0 /+- 0.6 fmol per milliliter (mean /+- SE). Slightly more than half of the CSF-SPLI cochromatographed with synthetic SP on Sephadex G-25. There was no apparent gradient in CSF-SPLI concentration over the first 30 ml of CSF removed by lumbar puncture. Mean concentrations CSF-SPLI in patients with Huntington disease, parkinsonism, miscellaneous dyskinesias, progressive supranuclear palsy, myopathy, and amyotrophic lateral sclerosis did not differ significantly from normal. Patients with neuropathy or multiple-system atrophy (Shy-Drager syndrome) had significantly reduced mean CSF-SPLI concentrations. These observations suggest that lumbar CSF-SPLI arises largely from spinal cord, nerve roots, or dorsal root ganglia, and that pathologic processes affecting these structures may be reflected by reduced levels of CSF-SPLI.
采用放射免疫分析法对人腰椎脑脊液（CSF）中的P物质（SP，一种假定的肽类神经递质）进行了测定。18名神经功能正常的成年人的脑脊液中存在P物质样免疫反应性（SPLI），其浓度范围为每毫升2.9至11.1飞摩尔，平均为每毫升7.0±0.6飞摩尔（平均值±标准误）。略多于一半的脑脊液SPLI与合成SP在葡聚糖G-25上共色谱分离。在通过腰椎穿刺抽取的最初30毫升脑脊液中，脑脊液SPLI浓度没有明显梯度。患有亨廷顿病、帕金森病、各种运动障碍、进行性核上性麻痹、肌病和肌萎缩侧索硬化症的患者的脑脊液SPLI平均浓度与正常情况无显著差异。患有神经病变或多系统萎缩（Shy-Drager综合征）的患者的脑脊液SPLI平均浓度显著降低。这些观察结果表明，腰椎脑脊液SPLI主要来源于脊髓、神经根或背根神经节，并且影响这些结构的病理过程可能通过脑脊液SPLI水平降低得以体现。
