Andersen H A, Lykkesfeldt A E, Nielsen S J
J Cell Sci. 1980 Oct;45:31-9. doi: 10.1242/jcs.45.1.31.
Tetrahymena cells secrete a factor which inhibits RNA synthesis in vivo and in vitro. The factor is a relatively small peptide with a molecular weight between 300 and 1500 Daltons. Other, non-specific peptides in the broth medium or added to a chemically defined medium have a stimulatory effect on RNA synthesis in vivo and such peptides also stimulate the in vitro synthesis of RNA in a r-chromatin preparation. On the basis of these results we conclude that such extracellular small peptides compete with a specific factor which is part of the intracellular regulatory mechanism controlling the rate of RNA synthesis. The consequence of such competition is a high overproduction of ribosomal RNA in cells inoculated on peptide-rich broth media.
四膜虫细胞分泌一种在体内和体外均能抑制RNA合成的因子。该因子是一种分子量在300至1500道尔顿之间的相对较小的肽。肉汤培养基中或添加到化学成分确定的培养基中的其他非特异性肽对体内RNA合成有刺激作用，并且这些肽也能刺激r-染色质制剂中RNA的体外合成。基于这些结果，我们得出结论，此类细胞外小肽与作为控制RNA合成速率的细胞内调节机制一部分的特定因子相互竞争。这种竞争的结果是接种在富含肽的肉汤培养基上的细胞中核糖体RNA大量过量产生。
