血小板的冷冻保存：四种方法的体外比较
Cryopreservation of platelets: an in-vitro comparison of four methods.
作者信息
Taylor M A
出版信息
J Clin Pathol. 1981 Jan;34(1):71-5. doi: 10.1136/jcp.34.1.71.
Abstract
Platelets were cryopreserved in four different cryoprotectives-two intracellular (dimethylsulphoxide, glycerol) and two extracellular (hydroxyethyl starch, dextran). The platelets were evaluated according to their yields, hypotonic shock response, and serotonin uptake, which are useful parameters for establishing optimum storage conditions. Hydroxyethyl starch and dextran were found to be poor cryoprotectives while 5% dimethylsulphoxide was the most suitable.
摘要
血小板在四种不同的冷冻保护剂中进行冷冻保存，其中两种为细胞内保护剂（二甲亚砜、甘油），两种为细胞外保护剂（羟乙基淀粉、右旋糖酐）。根据血小板的回收率、低渗休克反应和血清素摄取情况对其进行评估，这些都是确定最佳储存条件的有用参数。结果发现，羟乙基淀粉和右旋糖酐作为冷冻保护剂效果不佳，而5%的二甲亚砜是最合适的。
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