一种与私有 HLA-DR 抗原相关的“新的”致敏淋巴细胞分型（PLT）定义的 DP 抗原。

A "new" primed lymphocyte typing (PLT) defined DP-antigen associated with a private HLA--DR antigen.

作者信息

Morling N, Jakobsen B K, Platz P, Ryder L P, Svejgaard A, Thomsen M

出版信息

Tissue Antigens. 1980 Jul;16(1):95-104. doi: 10.1111/j.1399-0039.1980.tb00292.x.

DOI:10.1111/j.1399-0039.1980.tb00292.x

Abstract

We have recently described a "new" private HLA-DR antigen, DR"LTM", which has a frequency of approximately 0.6% in Danes. Primed Lymphocyte Typing (PLT) cells directed towards DR"LTM"-associated determinants were generated in vitro by haplotype primings in two unrelated families with DR"LTM" positive individuals. Both PLT-cells reacted in parallel and gave positive reactions with (i) two unrelated and (ii) eight related individuals, all of whom were DR"LTM"-positive. Both PLT-cells gave negative reactions with (i) 38 unrelated and (ii) 15 related DR"LTM"-negative individuals. Thus, there was total agreement between the results obtained by HLA-DR typing with the antiserum "LTM" and by PLT-typing with these two haplotype primed PLT-cells. None of the DP"LTM"-positive individuals carried more than one of the antigens HLA-Dw/-DRw/DP1-8 and the local specificity D/DP"H". Accordingly, this "new" PLT-defined antigen, DP"LTM", most probably belongs to the series of HLA-D/DR-associated DP-antigens previously described.

摘要

我们最近描述了一种“新的”私人 HLA - DR 抗原，即 DR“LTM”，在丹麦人群中的频率约为 0.6%。通过在两个具有 DR“LTM”阳性个体的无关家庭中进行单倍型激发，在体外产生了针对与 DR“LTM”相关决定簇的致敏淋巴细胞分型（PLT）细胞。两种 PLT 细胞平行反应，并与（i）两个无关个体和（ii）八个相关个体产生阳性反应，所有这些个体均为 DR“LTM”阳性。两种 PLT 细胞与（i）38 个无关的和（ii）15 个相关的 DR“LTM”阴性个体均产生阴性反应。因此，使用抗血清“LTM”进行 HLA - DR 分型以及使用这两种单倍型激发的 PLT 细胞进行 PLT 分型所获得的结果完全一致。没有一个 DP“LTM”阳性个体携带超过一种 HLA - Dw/-DRw/DP1 - 8 抗原和局部特异性 D/DP“H”。因此，这种“新的”由 PLT 定义的抗原 DP“LTM”很可能属于先前描述的一系列 HLA - D/DR 相关的 DP 抗原。