van Driel-Kulker A M, Ploem-Zaaijer J J, van der Zwan-van der Zwan M, Tanke H J
Anal Quant Cytol. 1980 Dec;2(4):243-6.
Quantitative cytology requires highly standardized preparation, fixation and staining techniques in order to obtain reproducible morphology (e.g., cell size, cell shape and chromatin distribution). We found centrifugal cytology best suited to this purpose. Therefore, we recently developed an improved bucket for centrifugation that permits sedimentation of cells in a fixative solution (2% polyethylene glycol in 50% ethanol) by using centrifugation at relatively high g forces. The cell quantity, the cell distribution and the flatness of the specimens thus prepared proved to be adequate for automated anlysis using the Leyden Television Analysis System (LEYTAS). Furthermore, different cytochemical and cytomorphologic staining procedures could be performed on different aliquots of the same cytologic sample without any change in the preparation or fixation technique.
定量细胞学需要高度标准化的制备、固定和染色技术，以便获得可重复的形态学特征（如细胞大小、细胞形状和染色质分布）。我们发现离心细胞学最适合这一目的。因此，我们最近开发了一种改进的离心桶，通过在相对较高的离心力下离心，使细胞在固定液（50%乙醇中的2%聚乙二醇）中沉降。结果证明，这样制备的标本的细胞数量、细胞分布和平整度足以使用莱顿电视分析系统（LEYTAS）进行自动分析。此外，对于同一细胞学样本的不同等分试样，可以进行不同的细胞化学和细胞形态学染色程序，而无需改变制备或固定技术。
